PMID- 25114044 OWN - NLM STAT- MEDLINE DCOM- 20141023 LR - 20140812 IS - 0177-5103 (Print) IS - 0177-5103 (Linking) VI - 110 IP - 3 DP - 2014 Aug 11 TI - Rearing practices identified as risk factors for ostreid herpesvirus 1 (OsHV-1) infection in Pacific oyster Crassostrea gigas spat. PG - 201-11 LID - 10.3354/dao02756 [doi] AB - Early detection of Pacific oyster spat infected with ostreid herpesvirus 1 (OsHV-1) could prevent introduction of OsHV-1-infected individuals into farming areas or onshore rearing facilities, thus reducing the risk of infection of naive oysters in such production systems. Experiments were conducted on several hundred oyster spat provided by producers in order to examine whether early rearing practices could be considered as potential risk factors for (1) OsHV-1 infection as detected by molecular methods and (2) spat mortality experimentally induced through thermal challenge. Spat groups collected on oyster beds and hatchery spat reared in growout areas during summer exhibited higher viral DNA contamination and mortalities during the trial than spat kept in onshore rearing facilities. Quantification of viral DNA before and during the trial showed that infection prevalence and intensity changed over time and revealed latent infection initially unsuspected in 3 of 10 groups. Thermal challenge induced a clear increase in the probability of detecting infected individuals, particularly for groups exhibiting significant prevalence of OsHV-1-contaminated spat prior to the challenge. The use of detection methods are discussed in relation to early rearing practices and disease control strategies. FAU - Normand, Julien AU - Normand J AD - Centre de Reference sur l'Huitre, Universite de Caen Basse Normandie, Caen, France. FAU - Blin, Jean-Louis AU - Blin JL FAU - Jouaux, Aude AU - Jouaux A LA - eng PT - Journal Article PL - Germany TA - Dis Aquat Organ JT - Diseases of aquatic organisms JID - 8807037 SB - IM MH - Animals MH - *Aquaculture MH - Crassostrea/*virology MH - Herpesviridae/*classification/*physiology MH - Host-Pathogen Interactions MH - Risk Factors EDAT- 2014/08/13 06:00 MHDA- 2014/10/24 06:00 CRDT- 2014/08/13 06:00 PHST- 2014/08/13 06:00 [entrez] PHST- 2014/08/13 06:00 [pubmed] PHST- 2014/10/24 06:00 [medline] AID - 10.3354/dao02756 [doi] PST - ppublish SO - Dis Aquat Organ. 2014 Aug 11;110(3):201-11. doi: 10.3354/dao02756.