PMID- 25116349
OWN - NLM
STAT- MEDLINE
DCOM- 20150413
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 34
IP  - 2
DP  - 2014
TI  - Parity and short-term estradiol treatment utilizes similar cellular mechanisms to 
      confer protection against breast cancer.
PG  - 491-505
LID - 10.1159/000363017 [doi]
AB  - BACKGROUND: Protective effect of early pregnancy and short-term estrogen 
      treatment (STET), against breast cancer is well established. The underlying 
      mechanisms are not well understood. In this study, we compared the mammary gland 
      cellular microenvironment influenced/induced by parity and STET alongside 
      age-matched controls. METHODS: Parous, STET, and control rats were injected with 
      N-methyl-N-nitrosourea at 15 weeks and monitored for the development of mammary 
      cancer. A subset of 4 rats were killed five weeks post carcinogen treatment and 
      mammary gland samples were isolated and subjected to molecular analysis. RESULTS: 
      Our results demonstrated a reduction in cell survival, extracellular matrix 
      associated proliferation, hormonal and growth factor receptor pathways in the 
      experimental groups compared to control rats. Moreover, concomitant reductions in 
      the EMT markers along with cell migration regulators were also observed in parous 
      and STET groups. Hormonal receptor such as GHR, PR, ERalpha and growth factor 
      receptors IGFR, EGFR and erbB2 were down regulated in the treatment groups. 
      Further analysis revealed that parity and STET drastically reduced the 
      expression, activation of JAK2 and nuclear localization of STATs. CONCLUSION: 
      Parity and STET by targeting major cell signaling pathways involved in cell 
      survival, cell migration and cell death reduces the mammary tumor promoting 
      environment.
CI  - (c) 2014 S. Karger AG, Basel.
FAU - Arumugam, Arunkumar
AU  - Arumugam A
AD  - Center of Excellence in Cancer Research, Department of Biomedical Sciences, Paul 
      L. Foster School of Medicine, Texas Tech University Health Sciences Center, El 
      Paso, TX, USA.
FAU - Subramani, Ramadevi
AU  - Subramani R
FAU - Nandy, Sushmita
AU  - Nandy S
FAU - Lopez, Rebecca
AU  - Lopez R
FAU - Boopalan, Thiyagarajan
AU  - Boopalan T
FAU - Lakshmanaswamy, Rajkumar
AU  - Lakshmanaswamy R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140808
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (STAT Transcription Factors)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.10.2 (Janus Kinases)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/enzymology/metabolism/*prevention & control
MH  - Estradiol/administration & dosage/*pharmacology
MH  - Female
MH  - Janus Kinases/metabolism
MH  - *Parity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - STAT Transcription Factors/metabolism
EDAT- 2014/08/15 06:00
MHDA- 2015/04/14 06:00
CRDT- 2014/08/14 06:00
PHST- 2014/06/10 00:00 [accepted]
PHST- 2014/08/14 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/04/14 06:00 [medline]
AID - 000363017 [pii]
AID - 10.1159/000363017 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2014;34(2):491-505. doi: 10.1159/000363017. Epub 2014 Aug 
      8.