PMID- 25117332
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20220408
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 3
DP  - 2014 Jul 29
TI  - Fibroin protein/chitosan scaffolds and bone marrow mesenchymal stem cells culture 
      in vitro.
PG  - 5745-53
LID - 10.4238/2014.July.29.1 [doi]
AB  - A previous experiment demonstrated that fibroin protein and chitosan mixed in 
      proper proportion presented good physical and chemical properties and biological 
      characteristics, which can make up for their respective disadvantages. To observe 
      the growth of bone marrow mesenchymal stem cells (BMSCs) on these fibroin 
      protein/chitosan 3D scaffolds, induced rabbit BMSCs were seeded on fibroin 
      protein/chitosan scaffolds. The cell adhesion rate was measured, and cell growth 
      was observed under an inverted microscope and a scanning electron microscope. The 
      cell adhesion rate increased with time. The inverted microscope observations 
      showed that the cells on fibroin protein/chitosan scaffolds could not be seen 
      clearly. As time passed, the number of cells around the stent increased and some 
      cells stretched inside the scaffolds. Electron microscopy showed active cell 
      growth and normal proliferation, and the granular and filamentous matrix 
      substances could be seen around cells. The microfilaments of cell and scaffold 
      materials were tightly connected. The cells not only grew on the surface of the 
      adherent material, but also stretched inside of the materials. These results 
      indicated that the fibroin protein/ chitosan mixed scaffolds have good 
      biocompatibility.
FAU - Deng, J
AU  - Deng J
AD  - Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, 
      Zunyi, Guizhou, China dj30666@126.com.
FAU - She, R F
AU  - She RF
AD  - Department of Orthopedics, Guizhou Provincial People's Hospital, Guiyang, 
      Guizhou, China.
FAU - Huang, W L
AU  - Huang WL
AD  - Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, 
      Zunyi, Guizhou, China.
FAU - Yuan, C
AU  - Yuan C
AD  - Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, 
      Zunyi, Guizhou, China.
FAU - Mo, G
AU  - Mo G
AD  - Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, 
      Zunyi, Guizhou, China.
LA  - eng
PT  - Journal Article
DEP - 20140729
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 9007-76-5 (Fibroins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - *Cell Culture Techniques
MH  - Chitosan/*chemistry
MH  - Female
MH  - Fibroins/*chemistry
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism/ultrastructure
MH  - Rabbits
MH  - Tissue Scaffolds/*chemistry
EDAT- 2014/08/15 06:00
MHDA- 2015/04/24 06:00
CRDT- 2014/08/14 06:00
PHST- 2014/08/14 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
AID - gmr3350 [pii]
AID - 10.4238/2014.July.29.1 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Jul 29;13(3):5745-53. doi: 10.4238/2014.July.29.1.