PMID- 25117363
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20140813
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 3
DP  - 2014 Aug 7
TI  - Effects of simulated weightlessness on cellular morphology and biological 
      characteristics of cell lines SGC-7901 and HFE-145.
PG  - 6060-9
LID - 10.4238/2014.August.7.20 [doi]
AB  - We investigated the effects of simulated weightlessness on cellular morphology, 
      proliferation, cell cycle, and apoptosis of the human gastric carcinoma cell line 
      SGC-7901 and the human gastric normal cell line HFE-145. A rotating clinostat was 
      used to simulate weightlessness. The Image-Pro4.5 image analysis system was used 
      for morphometric analysis. Proliferating cell nuclear antigen expression was 
      examined by immunohistochemical staining. Changes in the cell cycle were examined 
      using a cytometer. Apoptosis was measured using the terminal dUTP nick-end 
      labeling (TUNEL) method. When subjected to simulated weightlessness, the cellular 
      morphology of SGC-7901 cells was changed at 12, 24, 48, and 72 h, cell conversion 
      from the G1 to S phase was blocked, proliferation was inhibited at 48 and 72 h, 
      and the apoptosis index was increased at 72 h. The same changes were observed for 
      HFE-145 cells at 12 h when subjected to simulated weightlessness, but no 
      significant changes were found afterward compared with controls. SGC-7901 cells 
      change their cellular morphology and biological characteristics during 
      clinostat-simulated weightlessness at 72 h, but HFE-145 cells only change at 12 h 
      and adapt to simulated weightlessness after that point.
FAU - Zhu, M
AU  - Zhu M
AD  - Department of Gastroenterology, Air Force General Hospital of PLA, Beijing, China 
      mingzhucn@126.com.
FAU - Jin, X W
AU  - Jin XW
AD  - Department of Gastroenterology, Air Force General Hospital of PLA, Beijing, 
      China.
FAU - Wu, B Y
AU  - Wu BY
AD  - Department of Gastroenterology, South Building, Chinese PLA General Hospital, 
      Beijing, China.
FAU - Nie, J L
AU  - Nie JL
AD  - The 13th Laboratory of Institute of Space Medico-Engineering, Beijing, China.
FAU - Li, Y H
AU  - Li YH
AD  - The 13th Laboratory of Institute of Space Medico-Engineering, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20140807
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Adaptation, Biological
MH  - Apoptosis
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Proliferating Cell Nuclear Antigen/genetics/metabolism
MH  - Time Factors
MH  - *Weightlessness
EDAT- 2014/08/15 06:00
MHDA- 2015/04/24 06:00
CRDT- 2014/08/14 06:00
PHST- 2014/08/14 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
AID - gmr3654 [pii]
AID - 10.4238/2014.August.7.20 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Aug 7;13(3):6060-9. doi: 10.4238/2014.August.7.20.