PMID- 25117574
OWN - NLM
STAT- MEDLINE
DCOM- 20160128
LR  - 20211021
IS  - 1432-5195 (Electronic)
IS  - 0341-2695 (Linking)
VI  - 39
IP  - 1
DP  - 2015 Jan
TI  - Evaluation of intervertebral disc regeneration with implantation of bone marrow 
      mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits.
PG  - 149-59
LID - 10.1007/s00264-014-2481-0 [doi]
AB  - PURPOSE: The aim of the study was to investigate the curative effects of 
      transplantation of bone marrow mesenchymal stem cells (BMSCs) on intervertebral 
      disc regeneration and to investigate the feasibility of the quantitative T2 
      mapping method for evaluating repair of the nucleus pulposus after implantation 
      of BMSCs. METHODS: Forty-eight New Zealand white rabbits were used to establish 
      the lumber disc degenerative model by stabbing the annulus fibrosus and then 
      randomly divided into four groups, i.e. two weeks afterwards, BMSCs or 
      phosphate-buffered saline (PBS) were transplanted into degenerative discs (BMSCs 
      group and PBS group), while the operated rabbits without implantation of BMSCs or 
      PBS served as the sham group and the rabbits without operation were used as the 
      control group. At weeks two, six and ten after operation, the T2 values and disc 
      height indices (DHI) were calculated by magnetic resonance imaging (MRI 3.0 T), 
      and the gene expressions of type II collagen (COL2) and aggrecan (ACAN) in 
      degenerative discs were evaluated by real-time reverse transcription polymerase 
      chain reaction (RT-PCR). T2 values for the nucleus pulposus were correlated with 
      ACAN or COL2 expression by regression analysis. RESULTS: Cell clusters, 
      disorganised fibres, interlamellar glycosaminoglycan (GAG) matrix and 
      vascularisation were observed in lumber degenerative discs. BMSCs could be found 
      to survive in intervertebral discs and differentiate into nucleus pulposus-like 
      cells expressing COL2 and ACAN. The gene expression of COL2 and ACAN increased 
      during ten weeks after transplantation as well as the T2 signal intensity and T2 
      value. The DHI in the BMSCs group decreased more slowly than that in PBS and sham 
      groups. The T2 value correlated significantly with the gene expression of ACAN 
      and COL2 in the nucleus pulposus. CONCLUSIONS: Transplantation of BMSCs was able 
      to promote the regeneration of degenerative discs. Quantitative and non-invasive 
      T2 mapping could be used to evaluate the regeneration of the nucleus pulposus 
      with good sensitivity.
FAU - Cai, Feng
AU  - Cai F
AD  - Department of Orthopedics, Zhong Da Hospital, Southeast University, Nanjing, 
      China.
FAU - Wu, Xiao-Tao
AU  - Wu XT
FAU - Xie, Xin-Hui
AU  - Xie XH
FAU - Wang, Feng
AU  - Wang F
FAU - Hong, Xin
AU  - Hong X
FAU - Zhuang, Su-Yang
AU  - Zhuang SY
FAU - Zhu, Lei
AU  - Zhu L
FAU - Rui, Yun-Feng
AU  - Rui YF
FAU - Shi, Rui
AU  - Shi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140813
PL  - Germany
TA  - Int Orthop
JT  - International orthopaedics
JID - 7705431
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type II)
SB  - IM
MH  - Aggrecans/metabolism
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Collagen Type II/metabolism
MH  - Disease Models, Animal
MH  - Feasibility Studies
MH  - Intervertebral Disc/metabolism/*pathology/surgery
MH  - Intervertebral Disc Degeneration/*surgery
MH  - Magnetic Resonance Imaging/*methods
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction
MH  - Regeneration
EDAT- 2014/08/15 06:00
MHDA- 2016/01/29 06:00
CRDT- 2014/08/14 06:00
PHST- 2014/06/06 00:00 [received]
PHST- 2014/07/24 00:00 [accepted]
PHST- 2014/08/14 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2016/01/29 06:00 [medline]
AID - 10.1007/s00264-014-2481-0 [doi]
PST - ppublish
SO  - Int Orthop. 2015 Jan;39(1):149-59. doi: 10.1007/s00264-014-2481-0. Epub 2014 Aug 
      13.