PMID- 25120226
OWN - NLM
STAT- MEDLINE
DCOM- 20151204
LR  - 20211021
IS  - 2629-3277 (Electronic)
IS  - 2629-3277 (Linking)
VI  - 11
IP  - 1
DP  - 2015 Feb
TI  - Human adipose-derived mesenchymal stem cells improve motor functions and are 
      neuroprotective in the 6-hydroxydopamine-rat model for Parkinson's disease when 
      cultured in monolayer cultures but suppress hippocampal neurogenesis and 
      hippocampal memory function when cultured in spheroids.
PG  - 133-49
LID - 10.1007/s12015-014-9551-y [doi]
AB  - Adult human adipose-derived mesenchymal stem cells (MSC) have been reported to 
      induce neuroprotective effects in models for Parkinson's disease (PD). However, 
      these effects strongly depend on the most optimal application of the transplant. 
      In the present study we compared monolayer-cultured (aMSC) and spheroid (sMSC) 
      MSC following transplantation into the substantia nigra (SN) of 6-OHDA lesioned 
      rats regarding effects on the local microenvironment, degeneration of 
      dopaminergic neurons, neurogenesis in the hippocampal DG as well as motor and 
      memory function in the 6-OHDA-rat model for PD. aMSC transplantation 
      significantly increased tyrosine hydroxylase (TH) and brain-derived neurotrophic 
      factor (BDNF) levels in the SN, increased the levels of the glial fibrillary 
      acidic protein (GFAP) and improved motor functions compared to untreated and sMSC 
      treated animals. In contrast, sMSC grafting induced an increased local 
      microgliosis, decreased TH levels in the SN and reduced numbers of newly 
      generated cells in the dentate gyrus (DG) without yet affecting hippocampal 
      learning and memory function. We conclude that the neuroprotective potential of 
      adipose-derived MSC in the rat model of PD crucially depends on the applied 
      cellular phenotype.
FAU - Berg, Jurgen
AU  - Berg J
AD  - Department of Neurology, Charite University Medicine Berlin, CCM, Chariteplatz 1, 
      D-10117, Berlin, Germany.
FAU - Roch, Manfred
AU  - Roch M
FAU - Altschuler, Jennifer
AU  - Altschuler J
FAU - Winter, Christine
AU  - Winter C
FAU - Schwerk, Anne
AU  - Schwerk A
FAU - Kurtz, Andreas
AU  - Kurtz A
FAU - Steiner, Barbara
AU  - Steiner B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cell Rev Rep
JT  - Stem cell reviews and reports
JID - 101752767
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Adult
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Female
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Hippocampus/cytology/*physiology
MH  - Humans
MH  - Memory/*physiology
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/cytology/metabolism/*physiology
MH  - Microscopy, Confocal
MH  - Motor Activity/*physiology
MH  - Neurogenesis
MH  - Neuroprotection/physiology
MH  - Oxidopamine
MH  - Parkinsonian Disorders/chemically induced/*physiopathology/therapy
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transplantation, Heterologous
MH  - Tyrosine 3-Monooxygenase/genetics/metabolism
EDAT- 2014/08/15 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/08/15 06:00
PHST- 2014/08/15 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s12015-014-9551-y [doi]
PST - ppublish
SO  - Stem Cell Rev Rep. 2015 Feb;11(1):133-49. doi: 10.1007/s12015-014-9551-y.