PMID- 25120961
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140814
LR  - 20211021
IS  - 2249-782X (Print)
IS  - 0973-709X (Electronic)
IS  - 0973-709X (Linking)
VI  - 8
IP  - 6
DP  - 2014 Jun
TI  - Morphological study of dendritic cells in human cervix by zinc iodide osmium 
      method.
PG  - AC01-4
LID - 10.7860/JCDR/2014/7899.4436 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are a heterogeneous population of antigen 
      presenting cells that have been identified in several tissues including the 
      female reproductive organs. The aim of the present study is to demonstrate the 
      morphological differences of dendritic cells in normal human exocervix using the 
      Zinc Iodide Osmium (ZIO) procedure. MATERIALS AND METHODS: Normal cervical 
      tissues obtained from nine patients who underwent abdominal hysterectomies for 
      various ailments were processed for histochemical study. Six microns thick serial 
      sections were taken and viewed under a light microscope. The diameters of the 
      cells were measured under a magnification of 40x using the Cellsens image 
      analysing software and analysed using SPSS version 16. RESULTS AND CONCLUSION: In 
      the normal human exocervix, a greater density of ZIO-positive DCs was noted in 
      the epithelium and subepithelium and their distribution was not uniform. In some 
      areas of epithelium, the ZIO-positive cells in the basal layer showed a typical 
      dendritic morphology, while the cells in the intermediate and superficial layers 
      were nondendritic polygonal cells. Intraepithelial capillaries were noted, which 
      were surrounded by ZIO-positive nondendritic polygonal cells. There was 
      significant difference in the mean diameters of typical DCs (8.61+/-1.86 mum) and 
      nondendritic polygonal cells (10.97+/-1.93 mum). In the subepithelium the DCs had 
      typical morphology and their distribution varied. ZIO positive DCs were noted in 
      the epithelium and cervical glands of endocervix also. In conclusion, the human 
      cervix has different subsets of ZIO positive DCs with varied distribution. Their 
      functional role has yet to be defined.
FAU - Rabi, Suganthy
AU  - Rabi S
AD  - Professor, Department of Anatomy, Christian Medical College , Vellore, India .
FAU - Lionel, J
AU  - Lionel J
AD  - Professor, Department of Obstetrics & Gynaecology, Christian Medical College , 
      Vellore, India .
FAU - Indrasingh, Inbam
AU  - Indrasingh I
AD  - Professor, Department of Anatomy, Christian Medical College , Vellore, India .
LA  - eng
PT  - Journal Article
DEP - 20140620
PL  - India
TA  - J Clin Diagn Res
JT  - Journal of clinical and diagnostic research : JCDR
JID - 101488993
PMC - PMC4129312
OTO - NOTNLM
OT  - Dendritic cells
OT  - Human exocervix
OT  - Langerhans cells
OT  - Morphology
OT  - ZIO
EDAT- 2014/08/15 06:00
MHDA- 2014/08/15 06:01
PMCR- 2014/08/01
CRDT- 2014/08/15 06:00
PHST- 2013/10/14 00:00 [received]
PHST- 2014/05/01 00:00 [accepted]
PHST- 2014/08/15 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2014/08/15 06:01 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.7860/JCDR/2014/7899.4436 [doi]
PST - ppublish
SO  - J Clin Diagn Res. 2014 Jun;8(6):AC01-4. doi: 10.7860/JCDR/2014/7899.4436. Epub 
      2014 Jun 20.