PMID- 25120976
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140814
LR  - 20220317
IS  - 2249-782X (Print)
IS  - 0973-709X (Electronic)
IS  - 0973-709X (Linking)
VI  - 8
IP  - 6
DP  - 2014 Jun
TI  - Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in 
      Urinary Isolates, at a Tertiary Care Centre at Central India.
PG  - DC01-4
LID - 10.7860/JCDR/2014/7952.4413 [doi]
AB  - OBJECTIVE: To detect genes encoding carbapenem resistance in urinary isolates of 
      Escherichia coli recovered from hospitalized patients in tertiary care centre in 
      Pune, India. METHODS: From Jan 2012 to Dec 2012, a total of 300 consecutive 
      non-duplicate (one isolate per patient) clinical isolates of Escherichia coli 
      were recovered from urine cultures of hospitalized patients including hospital 
      acquired infection cases admitted to the medical and surgical intensive care 
      units. Polymerase chain reaction (PCR) assays and sequencing was used to 
      determine the presence of beta-lactamase encoding genes. Conjugation experiments 
      were performed to determine the transferability of beta-lactamase. RESULTS: All 
      the isolates were completely resistant to the second and third generation 
      cephalosporins tested as well as carbapenems. All the isolates showed 100% 
      susceptibility to tigecycline and colistin in vitro. Conjugation experiments 
      demonstrated that blaNDM-1 was transferable via plasmid. All the isolates showed 
      presence of blaNDM-1 and co-association of blaOXA-48 was 25/45(55%) of the 
      isolates. Repetitive element based PCR (REP PCR), Enterobacterial Repetitive 
      Intergenic Consensus (ERIC PCR) and Randomly Amplified Polymorphic DNA (RAPD) 
      revealed a diversity of six clonal types among E.coli isolates. CONCLUSION: 
      Co-production of NDM-1with OXA-48 in urinary isolates of E. coli was detected for 
      the first time in India. Transmission of plasmid carrying these resistant genes 
      to other members of Enterobacteriaceae will increase incidence of multidrug 
      resistance. Early detection of these genes will help in prevention and adequate 
      infection control by limiting the spread of these organisms.
FAU - Khajuria, Atul
AU  - Khajuria A
AD  - PhD Scholar, Department of Microbiology, Armed Forces Medical College , Pune, 
      India .
FAU - Praharaj, Ashok Kumar
AU  - Praharaj AK
AD  - PhD Scholar, Department of Microbiology, Armed Forces Medical College , Pune, 
      India .
FAU - Kumar, Mahadevan
AU  - Kumar M
AD  - Post Graduate, Department of Microbiology, Armed Forces Medical College , Pune, 
      India .
FAU - Grover, Naveen
AU  - Grover N
AD  - Post Graduate, Department of Microbiology, Armed Forces Medical College , Pune, 
      India .
LA  - eng
PT  - Journal Article
DEP - 20140620
PL  - India
TA  - J Clin Diagn Res
JT  - Journal of clinical and diagnostic research : JCDR
JID - 101488993
PMC - PMC4129265
OTO - NOTNLM
OT  - Beta-lactamase
OT  - Carbapenem resistance
OT  - Conjugation
OT  - Multidrug resistant
OT  - Uropathogenic E.coli
EDAT- 2014/08/15 06:00
MHDA- 2014/08/15 06:01
PMCR- 2014/08/01
CRDT- 2014/08/15 06:00
PHST- 2013/10/26 00:00 [received]
PHST- 2014/03/05 00:00 [accepted]
PHST- 2014/08/15 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2014/08/15 06:01 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.7860/JCDR/2014/7952.4413 [doi]
PST - ppublish
SO  - J Clin Diagn Res. 2014 Jun;8(6):DC01-4. doi: 10.7860/JCDR/2014/7952.4413. Epub 
      2014 Jun 20.