PMID- 25122057
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 24
IP  - 2
DP  - 2015 Jan 15
TI  - Effect of FGF-2 on collagen tissue regeneration by human vertebral bone marrow 
      stem cells.
PG  - 228-43
LID - 10.1089/scd.2014.0148 [doi]
AB  - The effects of fibroblast growth factor-2 (FGF-2) on collagen tissue regeneration 
      by human bone marrow stem cells (hBMSCs) were investigated. hBMSCs were isolated 
      from human vertebral body bone marrow during vertebral surgery and a population 
      of hBMSCs with the characteristics of mesenchymal stem cells was observed. The 
      FGF-2 treatment (5 ng/mL) affected on the colony-forming efficiency, 
      proliferation, and in vitro differentiation of hBMSCs. Insoluble/soluble collagen 
      and hydroxyproline synthesis was significantly enhanced in hBMSCs expanded with 
      FGF-2 and the treatment of FGF-2 caused a reduction in the mRNA expression of 
      collagen type I, but an increase of collagen types II and III along with lysyl 
      oxidase family genes. Collagen formation was also examined using an in vivo assay 
      model by transplanting hBMSCs into immunocompromised mice (n=4) and the 
      histologic and immunohistochemical results revealed that significantly more 
      collagen with a well-organized structure was formed by FGF-2-treated hBMSCs at 8 
      weeks posttransplantation (P<0.05). The DNA microarray assay demonstrated that 
      genes related to extracellular matrix formation were significantly upregulated. 
      To elucidate the underlying mechanism, chemical inhibitors against 
      extracellular-signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K) 
      were treated and following downstream expression was observed. Collectively, 
      FGF-2 facilitated the collagen-producing potency of hBMSCs both in vitro and in 
      vivo, rendering them more suitable for use in collagen regeneration in the 
      clinical field.
FAU - Park, Dong-Soo
AU  - Park DS
AD  - 1 Department of Periodontology, Research Institute for Periodontal Regeneration, 
      College of Dentistry, Yonsei University , Seoul, Korea.
FAU - Park, Jung-Chul
AU  - Park JC
FAU - Lee, Jung-Seok
AU  - Lee JS
FAU - Kim, Tae-Wan
AU  - Kim TW
FAU - Kim, Ki-Joon
AU  - Kim KJ
FAU - Jung, Byung-Joo
AU  - Jung BJ
FAU - Shim, Eun-Kyung
AU  - Shim EK
FAU - Choi, Eun-Young
AU  - Choi EY
FAU - Park, So-Yon
AU  - Park SY
FAU - Cho, Kyoo-Sung
AU  - Cho KS
FAU - Kim, Chang-Sung
AU  - Kim CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.4.3.13 (Protein-Lysine 6-Oxidase)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Collagen/*biosynthesis
MH  - Female
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Heterografts
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Mice, SCID
MH  - Middle Aged
MH  - Protein-Lysine 6-Oxidase/biosynthesis
MH  - Regeneration/*drug effects
MH  - Spine/cytology/*metabolism
EDAT- 2014/08/15 06:00
MHDA- 2015/09/17 06:00
CRDT- 2014/08/15 06:00
PHST- 2014/08/15 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - 10.1089/scd.2014.0148 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2015 Jan 15;24(2):228-43. doi: 10.1089/scd.2014.0148.