PMID- 2512352
OWN - NLM
STAT- MEDLINE
DCOM- 19900119
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 143
IP  - 12
DP  - 1989 Dec 15
TI  - Mouse antibody response to group A streptococcal carbohydrate.
PG  - 4213-20
AB  - In an attempt to more fully understand the generation of antibody diversity to 
      carbohydrate (CHO) Ag, we produced and characterized a panel of hybridoma cell 
      lines specific for group A streptococcal CHO from mice injected with the intact 
      bacteria (minus the hyaluronic acid capsule and cell wall protein Ag). We have 
      analyzed the use of H and L chain V region genes in the early (day 7) and late 
      response (hyperimmune) and have sequenced the dominant VH gene used in several of 
      our hybridomas. Our data allowed us to assess the extent to which the 
      recombination of various V, D, and J gene segments and somatic mutation 
      contribute to antibody diversification in this system. In this report we confirm 
      that a minimum of two VH and four VK gene segments are used to encode this 
      response. We extend this analysis to show that multiple D and J gene segments are 
      used and that a significant amount of junctional variability is tolerated in CDR 
      3. Our results indicate that the level of somatic mutation in the hyperimmune 
      response is generally low in comparison with the response to haptens and protein 
      Ag. These data also suggest that there is a positive selection for mutation in 
      CDR 1 during the hyperimmune response to group A streptococcal CHO.
FAU - Jarvis, C D
AU  - Jarvis CD
AD  - Department of Molecular Genetics and Microbiology, University of Massachusetts 
      Medical School, Worcester 01655.
FAU - Cannon, L E
AU  - Cannon LE
FAU - Stavnezer, J
AU  - Stavnezer J
LA  - eng
GR  - AI-23283/AI/NIAID NIH HHS/United States
GR  - CA-32497/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Immunoglobulin kappa-Chains)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (streptococcal group A cell wall carbohydrate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Bacterial/*biosynthesis
MH  - Base Sequence
MH  - Cell Line
MH  - Genes, Immunoglobulin
MH  - Hybridomas/metabolism
MH  - Immunoglobulin Heavy Chains/biosynthesis/genetics
MH  - Immunoglobulin Variable Region/biosynthesis/genetics
MH  - Immunoglobulin kappa-Chains/biosynthesis/genetics
MH  - Mice
MH  - Mice, Inbred A
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Polysaccharides, Bacterial/*immunology
MH  - Streptococcus pyogenes/*immunology
MH  - T-Lymphocytes/metabolism
EDAT- 1989/12/15 00:00
MHDA- 1989/12/15 00:01
CRDT- 1989/12/15 00:00
PHST- 1989/12/15 00:00 [pubmed]
PHST- 1989/12/15 00:01 [medline]
PHST- 1989/12/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1989 Dec 15;143(12):4213-20.