PMID- 25123565
OWN - NLM
STAT- MEDLINE
DCOM- 20150519
LR  - 20140911
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 81
IP  - 9
DP  - 2014 Sep
TI  - Evidence for a novel, local acute-phase response in the bovine oviduct: 
      progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein 
      expression in epithelial cells in vitro.
PG  - 861-70
LID - 10.1002/mrd.22355 [doi]
AB  - Little is known about the local production and function of alpha 1-acid 
      glycoprotein (AGP), an acute-phase protein, in the female reproductive tract. 
      This study aimed to investigate the regulation and immune function of AGP in 
      cultured bovine oviduct epithelial cells. Analysis by Western blotting and 
      immunohistochemistry revealed that bovine oviduct tissue expresses AGP protein in 
      epithelial cells and the smooth muscle layer. Stimulation of bovine oviduct 
      epithelial cells in culture with either progesterone (1 ng/ml) or 
      lipopolysaccharide (LPS, 10 ng/ml) induced both mRNA expression and secretion of 
      AGP. Estradiol (1 ng/ml), progesterone (1 ng/ml), and luteinizing hormone 
      (10 ng/ml), which are observed during the peri-ovulatory period in oviduct 
      tissues (steroids) or in circulation (luteinizing hormone), suppressed 
      LPS-induced expression and secretion of AGP, which in turn induced the expression 
      of Toll-like receptor-4 (TLR-4) and interleukin-1beta (IL-1B), but suppressed TLR-2 
      and tumor necrosis factor-alpha (TNFA) expression. AGP also inhibited LPS-induced 
      TLR-2 and TNFA expression, but had no effect on LPS-induced TLR-4 and IL-1B 
      expression. These findings suggest that oviductal epithelial cells can 
      participate in antimicrobial processes through the secretion of AGP, which is 
      partly regulated by ovarian steroids. Moreover, oviductal AGP may regulate the 
      response of epithelial cells, thereby reducing the expression of the acute 
      pro-inflammatory cytokine TNFA, which could contribute to the local homeostasis 
      during the acute response to endotoxin release in the oviduct's anti-infection 
      process.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Kowsar, R
AU  - Kowsar R
AD  - Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and 
      Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
FAU - Hambruch, N
AU  - Hambruch N
FAU - Marey, M A
AU  - Marey MA
FAU - Liu, J
AU  - Liu J
FAU - Shimizu, T
AU  - Shimizu T
FAU - Pfarrer, C
AU  - Pfarrer C
FAU - Miyamoto, A
AU  - Miyamoto A
LA  - eng
PT  - Journal Article
DEP - 20140813
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Orosomucoid)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Epithelial Cells/cytology/drug effects/metabolism
MH  - Fallopian Tubes/*drug effects/*metabolism
MH  - Female
MH  - Lipopolysaccharides/*pharmacology
MH  - Orosomucoid/*metabolism
MH  - Progesterone/*pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2014/08/16 06:00
MHDA- 2015/05/20 06:00
CRDT- 2014/08/16 06:00
PHST- 2013/10/11 00:00 [received]
PHST- 2014/06/17 00:00 [accepted]
PHST- 2014/08/16 06:00 [entrez]
PHST- 2014/08/16 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - 10.1002/mrd.22355 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2014 Sep;81(9):861-70. doi: 10.1002/mrd.22355. Epub 2014 Aug 13.