PMID- 25124708
OWN - NLM
STAT- MEDLINE
DCOM- 20150624
LR  - 20220409
IS  - 1872-7654 (Electronic)
IS  - 0301-2115 (Linking)
VI  - 181
DP  - 2014 Oct
TI  - Vitamin C attenuates detrimental effects of diabetes mellitus on sperm 
      parameters, chromatin quality and rate of apoptosis in mice.
PG  - 32-6
LID - S0301-2115(14)00379-0 [pii]
LID - 10.1016/j.ejogrb.2014.07.007 [doi]
AB  - OBJECTIVES: The main goal was to examine the protective effects of vitamin C on 
      sperm parameters, sperm chromatin condensation and apoptosis in 
      experimentally-induced diabetic mice. STUDY DESIGN: 28 adult Syrian mice were 
      divided into 4 groups. In Group1, the mice were diabetic that received a single 
      dose of Streptozocin (STZ) (200mg/kg) intra-peritoneally (ip). Group 2 was 
      included diabetic mice that received vitamin C (10mg/kg/daily, ip). Mice in group 
      3 were received vitamin C and group 4 was considered as control. After 35 days, 
      sperm analysis was done accordingly. To asses sperm chromatin and DNA quality, we 
      used aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), acridine 
      orange (AO) and terminal transferase mediated deoxyuridine triphosphate biotin 
      end labeling (TUNEL) tests. RESULTS: All of the sperm parameters (count, 
      motility, morphology and viability) had significant reduction in diabetic mice 
      but, the data showed a significant increase in all of the sperm parameters in 
      diabetic+vitamin C when compared with diabetic and control animals (P<0.05). 
      There were significant differences (P<0.001) between groups regarding to TB 
      staining (48.8+/-5.92 vs 34.3+/-4.13), AO test (35.9+/-6.11 vs 20.8+/-2.89) and TUNEL 
      test (39.42+/-7.18 vs 22.00+/-3.65) in diabetic and diabetic+vitamin C groups, 
      respectively. Nevertheless, in CMA3 and AB staining assays, there were not any 
      significant differences between different groups. CONCLUSION: Vitamin C, as a 
      potent antioxidant, can attenuate detrimental effects of diabetes mellitus on the 
      sperm parameters, chromatin quality and apoptosis in an experimental model.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Research & Clinical Center for Infertility, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
FAU - Mangoli, Esmat
AU  - Mangoli E
AD  - Research & Clinical Center for Infertility, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran. Electronic address: es.mangoli@gmail.com.
FAU - Nahangi, Hossein
AU  - Nahangi H
AD  - Department of Biology & Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Anvari, Morteza
AU  - Anvari M
AD  - Department of Biology & Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Pourentezari, Majid
AU  - Pourentezari M
AD  - Department of Biology & Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Halvaei, Iman
AU  - Halvaei I
AD  - Research & Clinical Center for Infertility, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140730
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (Antioxidants)
RN  - 0 (Chromatin)
RN  - 5W494URQ81 (Streptozocin)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/*therapeutic use
MH  - Apoptosis/*drug effects
MH  - Ascorbic Acid/*therapeutic use
MH  - *Chromatin/drug effects
MH  - Diabetes Mellitus, Experimental/chemically induced/*complications
MH  - Male
MH  - Mice
MH  - *Semen Analysis
MH  - Spermatozoa/*drug effects
MH  - Streptozocin
OTO - NOTNLM
OT  - Apoptosis
OT  - Chromatin
OT  - Diabetes mellitus
OT  - Mice
OT  - Sperm
OT  - Vitamin C
EDAT- 2014/08/16 06:00
MHDA- 2015/06/25 06:00
CRDT- 2014/08/16 06:00
PHST- 2014/01/06 00:00 [received]
PHST- 2014/05/28 00:00 [revised]
PHST- 2014/07/20 00:00 [accepted]
PHST- 2014/08/16 06:00 [entrez]
PHST- 2014/08/16 06:00 [pubmed]
PHST- 2015/06/25 06:00 [medline]
AID - S0301-2115(14)00379-0 [pii]
AID - 10.1016/j.ejogrb.2014.07.007 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2014 Oct;181:32-6. doi: 
      10.1016/j.ejogrb.2014.07.007. Epub 2014 Jul 30.