PMID- 25125146
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20211021
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 38
IP  - 1
DP  - 2015 Feb
TI  - Relevance of the myeloid differentiation factor 88 (MyD88) on RANKL, OPG, and nod 
      expressions induced by TLR and IL-1R signaling in bone marrow stromal cells.
PG  - 1-8
LID - 10.1007/s10753-014-0001-4 [doi]
AB  - The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like 
      receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We 
      examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and 
      nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappaB) activation 
      and nucleotide-binding oligomerization domain (Nod) induction by 
      lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of 
      NF-kappaB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal 
      cell (BMSC). RANKL, Nod1, Nod2, NF-kappaB, and p38 protein levels were determined by 
      Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 
      stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after 
      LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 
      was necessary for NF-kappaB and p38 MAPK activation. MDP alone did not induce RANKL 
      and OPG expressions; however, when combined with LPS, their expressions were 
      significantly increased (p < 0.05). Our results support that MyD88 signaling has 
      a pivotal role in osteoclastogenesis thought NF-kappaB and p38 activation. Nod2 and 
      especially Nod1 levels were influenced by MyD88.
FAU - Leite, Fabio Renato Manzolli
AU  - Leite FR
AD  - School of Dentistry, Federal University at Pelotas (UFPel), Pelotas, RS, Brazil.
FAU - de Aquino, Sabrina Garcia
AU  - de Aquino SG
FAU - Guimaraes, Morgana Rodrigues
AU  - Guimaraes MR
FAU - Cirelli, Joni Augusto
AU  - Cirelli JA
FAU - Zamboni, Dario S
AU  - Zamboni DS
FAU - Silva, Joao S
AU  - Silva JS
FAU - Junior, Carlos Rossa
AU  - Junior CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NOD1 protein, human)
RN  - 0 (NOD2 protein, human)
RN  - 0 (Nod1 Signaling Adaptor Protein)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (TNFRSF11B protein, human)
RN  - 0 (Tnfsf11 protein, mouse)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myeloid Differentiation Factor 88/*biosynthesis
MH  - Nod1 Signaling Adaptor Protein/biosynthesis
MH  - Nod2 Signaling Adaptor Protein/biosynthesis
MH  - Osteoprotegerin/*biosynthesis
MH  - RANK Ligand/*biosynthesis
MH  - Receptors, Interleukin-1/*biosynthesis
MH  - Signal Transduction/physiology
MH  - Toll-Like Receptors/*biosynthesis
EDAT- 2014/08/16 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/08/16 06:00
PHST- 2014/08/16 06:00 [entrez]
PHST- 2014/08/16 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s10753-014-0001-4 [doi]
PST - ppublish
SO  - Inflammation. 2015 Feb;38(1):1-8. doi: 10.1007/s10753-014-0001-4.