PMID- 25125622
OWN - NLM
STAT- MEDLINE
DCOM- 20141017
LR  - 20140815
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 142
IP  - 3
DP  - 2014 Sep
TI  - A dual-fixed neutrophil substrate improves interpretation of antineutrophil 
      cytoplasmic antibodies by indirect immunofluorescence.
PG  - 325-30
LID - 10.1309/AJCPG02FGQVAUSIU [doi]
AB  - OBJECTIVES: To determine whether the addition of a formalin-fixed neutrophil 
      substrate could improve interpretation and prediction of autoantigenic 
      specificity in antineutrophil cytoplasmic antibody (ANCA) testing. METHODS: 
      Routine diagnostic samples sent for ANCA testing were analyzed prospectively on a 
      dual substrate of both ethanol- and formalin-fixed neutrophils. Positive samples 
      on ethanol-fixed neutrophils were deemed "typical" if formalin-fixed neutrophils 
      also stained, and "atypical" if not. Indirect immunofluorescence (IIF) results 
      were correlated with antimyeloperoxidase (MPO) and anti-proteinase 3 (PR3) 
      results with an enzyme-linked immunosorbent assay (ELISA). RESULTS: Of 1,426 
      samples, 201 from unique patients were ANCA-positive (200 on IIF, 1 on ELISA 
      alone). Thirty-two (45%) of 71 typical ANCA staining patterns were positive for 
      either an anti-MPO or anti-PR3 antibodies, whereas only one (0.8%) of 129 
      atypical patterns was ELISA-positive, in a patient without systemic vasculitis. 
      Only one (3%) of 34 ELISA-positive samples had a negative IIF-ANCA (1/1,426 
      patients, 0.07%), and this patient did not have vasculitis. CONCLUSIONS: 
      Concomitant staining on formalin fixation of IIF-positive ethanol-fixed ANCA 
      samples improves the interpretation of ANCA testing and is predictive of 
      vasculitis autoantigens MPO and PR3.
CI  - Copyright(c) by the American Society for Clinical Pathology.
FAU - Lin, Ming-Wei
AU  - Lin MW
AD  - From the Department of Immunopathology, Pathology West, ICPMR, Westmead Hospital, 
      Westmead, Australia, and Discipline of Medicine, Sydney Medical School, 
      University of Sydney, Sydney, Australia. ming-wei.lin@sydney.edu.au.
FAU - Silvestrini, Roger A
AU  - Silvestrini RA
AD  - From the Department of Immunopathology, Pathology West, ICPMR, Westmead Hospital, 
      Westmead, Australia, and.
FAU - Culican, Suzanne
AU  - Culican S
AD  - From the Department of Immunopathology, Pathology West, ICPMR, Westmead Hospital, 
      Westmead, Australia, and.
FAU - Campbell, David
AU  - Campbell D
AD  - From the Department of Immunopathology, Pathology West, ICPMR, Westmead Hospital, 
      Westmead, Australia, and.
FAU - Fulcher, David A
AU  - Fulcher DA
AD  - From the Department of Immunopathology, Pathology West, ICPMR, Westmead Hospital, 
      Westmead, Australia, and Discipline of Medicine, Sydney Medical School, 
      University of Sydney, Sydney, Australia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/*diagnosis/immunology
MH  - Antibodies, Antineutrophil Cytoplasmic/*analysis
MH  - Ethanol
MH  - Fluorescent Antibody Technique, Indirect/*methods
MH  - Formaldehyde
MH  - Humans
MH  - Neutrophils/*immunology
OTO - NOTNLM
OT  - Antineutrophil cytoplasmic antibody (ANCA)
OT  - Formalin fixation
OT  - Indirect immunofluorescence (IIF)
OT  - Vasculitis
EDAT- 2014/08/16 06:00
MHDA- 2014/10/18 06:00
CRDT- 2014/08/16 06:00
PHST- 2014/08/16 06:00 [entrez]
PHST- 2014/08/16 06:00 [pubmed]
PHST- 2014/10/18 06:00 [medline]
AID - 142/3/325 [pii]
AID - 10.1309/AJCPG02FGQVAUSIU [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2014 Sep;142(3):325-30. doi: 10.1309/AJCPG02FGQVAUSIU.