PMID- 25126835
OWN - NLM
STAT- MEDLINE
DCOM- 20150515
LR  - 20181202
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 15
IP  - 9
DP  - 2014 Sep 8
TI  - Inhibition of protein and cell attachment on materials generated from 
      N-(2-hydroxypropyl) acrylamide.
PG  - 3259-66
LID - 10.1021/bm500654q [doi]
AB  - Effective control over biointerfacial interactions is essential for a broad range 
      of biomedical applications. At this point in time, only a relatively small range 
      of radically polymerizable monomers have been described that are able to generate 
      low fouling polymer materials and surfaces. The most important examples that have 
      been successfully used in the context of the reduction of nonspecific protein 
      adsorption and subsequent cell attachment include PEG-based monomers such as 
      poly(ethylene glycol) methacrylate (PEGMA), zwitterionic monomers such as 
      2-methacryloyloxyethyl phosphorylcholine and noncharged monomers such as 
      acrylamide and N-(2-hydroxypropyl) methacrylamide (HPMAm). However, issues such 
      as oxidative degradation and poor polymerization characteristics limit the 
      applicability of most of these candidates. Here we have synthesized the monomer 
      N-(2-hydroxypropyl) acrylamide (HPAm), examined its polymerization kinetics and 
      evaluated its suitability for RAFT mediated polymerization in comparison to 
      HPMAm. We also synthesized hydrogels using HPMAm and HPAm and evaluated the 
      ability of HPAm polymers to occlude protein adsorption and cell attachment. In 
      RAFT-controlled polymerization, much faster (8x) polymerization was observed for 
      HPAm relative to HPMAm and better control was achieved over the molecular weight 
      distribution. The performance of hydrogels prepared from HPAm in the prevention 
      of protein adsorption and cellular attachment was equivalent to or better than 
      that observed for materials made from HPMAm and PEG. These results open the door 
      for HPAm based polymers in applications where effective control over 
      biointerfacial interactions is required.
FAU - Fairbanks, Benjamin D
AU  - Fairbanks BD
AD  - CSIRO Manufacturing Flagship, Bayview Avenue, Clayton 3169 VIC, Australia.
FAU - Thissen, Helmut
AU  - Thissen H
FAU - Maurdev, George
AU  - Maurdev G
FAU - Pasic, Paul
AU  - Pasic P
FAU - White, Jacinta F
AU  - White JF
FAU - Meagher, Laurence
AU  - Meagher L
LA  - eng
PT  - Journal Article
DEP - 20140827
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Hydrogels)
RN  - 0 (Methacrylates)
RN  - 0 (Proteins)
RN  - 0 (polyethylene glycol methacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - UKW89XAX2X (hydroxypropyl methacrylate)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Fibroblasts/cytology/*metabolism
MH  - *Hydrogels/chemical synthesis/chemistry/pharmacology
MH  - *Methacrylates/chemical synthesis/chemistry/pharmacology
MH  - Mice
MH  - Oxidation-Reduction
MH  - *Polyethylene Glycols/chemical synthesis/chemistry/pharmacology
MH  - Proteins/*chemistry
EDAT- 2014/08/16 06:00
MHDA- 2015/05/16 06:00
CRDT- 2014/08/16 06:00
PHST- 2014/08/16 06:00 [entrez]
PHST- 2014/08/16 06:00 [pubmed]
PHST- 2015/05/16 06:00 [medline]
AID - 10.1021/bm500654q [doi]
PST - ppublish
SO  - Biomacromolecules. 2014 Sep 8;15(9):3259-66. doi: 10.1021/bm500654q. Epub 2014 
      Aug 27.