PMID- 2512688
OWN - NLM
STAT- MEDLINE
DCOM- 19900125
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 56
IP  - 1
DP  - 1989 Oct 1
TI  - Interaction of thrombin with heparin cofactor II and antithrombin III on 
      prostacyclin production by cultured endothelial cells.
PG  - 99-107
AB  - To investigate the physiologic function of heparin cofactor II (HCII), 
      endothelial cells from human umbilical vein were incubated in vitro for 20 min 
      with 0.5 NIH U/ml thrombin in the presence of HCII or antithrombin III (ATIII), 
      and prostacyclin production determined by radioimmunoassay for 
      6-keto-prostaglandin F1 alpha, the stable metabolite of prostacyclin. Although 
      ATIII at 20 mInh.U/ml slightly but significantly inhibited thrombin-induced 
      prostacyclin production, neither unfractionated heparin (UFH) nor low molecular 
      weight heparin (LMWH) at 1 U/ml accelerated the inhibitory effect of ATIII. HCII 
      at 10 and 20 mInh.U/ml did not decrease thrombin stimulation of prostacyclin 
      production in the presence or absence of UFH or LMWH. However, HCII caused a 
      marked decrease in the thrombin-stimulated prostacyclin prostacyclin production 
      in the presence of 2 mg/ml dermatan sulfate (DS). The significant inhibition by 
      HCII occurred when the DS concentrations were 0.2 microgram/ml and higher. From 
      these results we suggest that HCII may prevent a prostacyclin-induced inhibition 
      of platelet aggregation for hemostasis when plasma is exposed to vascular smooth 
      muscle cells or fibroblasts which synthesize a significant amount of DS.
FAU - Kaji, T
AU  - Kaji T
AD  - Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical 
      and Pharmaceutical University, Japan.
FAU - Itoh, F
AU  - Itoh F
FAU - Hayakawa, Y
AU  - Hayakawa Y
FAU - Oguma, Y
AU  - Oguma Y
FAU - Sakuragawa, N
AU  - Sakuragawa N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 81604-65-1 (Heparin Cofactor II)
RN  - 9000-94-6 (Antithrombin III)
RN  - 9005-49-6 (Heparin)
RN  - DCR9Z582X0 (Epoprostenol)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Antithrombin III/*pharmacology
MH  - Cells, Cultured
MH  - Dermatan Sulfate/pharmacology
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Epoprostenol/*biosynthesis
MH  - Heparin/pharmacology
MH  - Heparin Cofactor II/*pharmacology
MH  - Humans
MH  - Thrombin/*pharmacology
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
AID - 10.1016/0049-3848(89)90012-1 [doi]
PST - ppublish
SO  - Thromb Res. 1989 Oct 1;56(1):99-107. doi: 10.1016/0049-3848(89)90012-1.