PMID- 25128322
OWN - NLM
STAT- MEDLINE
DCOM- 20151020
LR  - 20220321
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 278
DP  - 2014 Oct 10
TI  - Infusion of Gabralpha6 siRNA into the trigeminal ganglia increased the myogenic 
      orofacial nociceptive response of ovariectomized rats treated with 17beta-estradiol.
PG  - 144-53
LID - S0306-4522(14)00639-3 [pii]
LID - 10.1016/j.neuroscience.2014.07.066 [doi]
AB  - High levels of 17beta-estradiol (E2) have been found to reduce inflammatory 
      temporomandibular joint (TMJ) pain. A search for genes effected by a high 
      concentration of estradiol showed an increase in GABAA receptor subunit alpha 6 
      (Gabralpha6) in the trigeminal ganglia (TG). Blockade of Gabralpha6 expression in the TG 
      increases masseter muscle nociception in male rats, but the relationship between 
      estradiol's effect on nociception and Gabralpha6 expression remains unclear in 
      females. To address this knowledge gap we hypothesized that reducing Gabralpha6 
      expression in the TG will increase the orofacial nociceptive response of 
      ovariectomized female rats treated with estradiol. To administer hormone osmotic 
      pumps were placed in rats that dispensed a low diestrus plasma concentration of 
      17beta-estradiol, in addition, 17beta-estradiol was injected to produce a high 
      proestrus plasma concentration of estradiol. A ligature was then placed around 
      the masseter tendon to induce a nociceptive response; a model for TMJ muscle 
      pain. Gabralpha6 small interfering RNA (siRNA) was later infused into the TG and the 
      nociceptive response was measured using von Frey filaments and a meal duration 
      assay. GABAA receptor expression was measured in the TG and trigeminal nucleus 
      caudalis and upper cervical region (Vc-C1). Ligature significantly increased the 
      nociceptive response but a high proestrus concentration of 17beta-estradiol 
      attenuated this response. Gabralpha6 siRNA infusion decreased Gabralpha6 expression in 
      the TG and Vc-C1 but increased the nociceptive response after 17beta-estradiol 
      treatment. The results suggest estradiol decreased the orofacial nociceptive 
      response, in part, by causing an increase in Gabralpha6 expression.
CI  - Copyright (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Kramer, P R
AU  - Kramer PR
AD  - Department of Biomedical Sciences, Texas A&M University, Baylor College of 
      Dentistry, 3302 Gaston Avenue, Dallas, TX 75246, United States. Electronic 
      address: pkramer@bcd.tamhsc.edu.
FAU - Bellinger, L L
AU  - Bellinger LL
AD  - Department of Biomedical Sciences, Texas A&M University, Baylor College of 
      Dentistry, 3302 Gaston Avenue, Dallas, TX 75246, United States.
LA  - eng
GR  - DE022129/DE/NIDCR NIH HHS/United States
GR  - R01 DE022129/DE/NIDCR NIH HHS/United States
GR  - R01 DE016059/DE/NIDCR NIH HHS/United States
GR  - DE016059/DE/NIDCR NIH HHS/United States
GR  - R01 DE015372/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140812
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, GABA-A)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Estradiol/pharmacology/*physiology
MH  - Estrous Cycle/drug effects
MH  - Female
MH  - Nociception/*physiology
MH  - Ovariectomy
MH  - Pain Measurement
MH  - Protein Subunits/biosynthesis
MH  - RNA, Small Interfering/administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA-A/*biosynthesis
MH  - Temporomandibular Joint/metabolism
MH  - Trigeminal Ganglion/drug effects/*metabolism
PMC - PMC4172543
MID - NIHMS622485
OTO - NOTNLM
OT  - estradiol
OT  - nociception
OT  - pain
OT  - temporomandibular joint
OT  - temporomandibular joint disorders
EDAT- 2014/08/17 06:00
MHDA- 2015/10/21 06:00
PMCR- 2015/10/10
CRDT- 2014/08/17 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2014/07/29 00:00 [revised]
PHST- 2014/07/30 00:00 [accepted]
PHST- 2014/08/17 06:00 [entrez]
PHST- 2014/08/17 06:00 [pubmed]
PHST- 2015/10/21 06:00 [medline]
PHST- 2015/10/10 00:00 [pmc-release]
AID - S0306-4522(14)00639-3 [pii]
AID - 10.1016/j.neuroscience.2014.07.066 [doi]
PST - ppublish
SO  - Neuroscience. 2014 Oct 10;278:144-53. doi: 10.1016/j.neuroscience.2014.07.066. 
      Epub 2014 Aug 12.