PMID- 25129550
OWN - NLM
STAT- MEDLINE
DCOM- 20151203
LR  - 20200930
IS  - 1873-2690 (Electronic)
IS  - 0981-9428 (Linking)
VI  - 83
DP  - 2014 Oct
TI  - alpha-linolenic acid concentration and not wounding per se is the key regulator of 
      octadecanoid (oxylipin) pathway activity in rice (Oryza sativa L.) leaves.
PG  - 117-25
LID - S0981-9428(14)00233-2 [pii]
LID - 10.1016/j.plaphy.2014.07.013 [doi]
AB  - Using an in vitro system composed of crushed leaf tissues to simulate the 
      wounding response in rice leaves, we established that synthesis of jasmonic acid 
      (JA) and jasmonic acid-isoleucine (JA-Ile) can only occur in unwounded tissue 
      and, in wounded tissue, that only the chloroplast-located section of the 
      octadecanoid pathway is active, resulting in the accumulation of 
      12-oxo-phytodienoic acid (OPDA). We further showed that OPDA accumulation 
      in vitro was inhibited by 90% using the general lipase inhibitor, 
      tetrahydrolipstatin, indicating that production of alpha-linolenic acid was the 
      rate-limiting step in octadecanoid pathway activity in rice following wounding 
      and the enzyme capacity for an active pathway was already present. We confirmed 
      this result by showing that added alpha-linolenic acid stimulated OPDA synthesis 
      in vitro and stimulated OPDA, JA and JA-Ile synthesis in vivo in unwounded 
      tissue. Thus, the response to wounding can be mimicked by the provision of free 
      alpha-linolenic acid. Our results draw attention to the key importance of lipase 
      activity in initiation of JA and JA-Ile biosynthesis and our lack of knowledge of 
      the cognate lipase(s), lipase substrate identity and mechanism(s) of activation 
      in wounded and unwounded tissue.
CI  - Copyright (c) 2014 Elsevier Masson SAS. All rights reserved.
FAU - Christeller, John T
AU  - Christeller JT
AD  - Institute of Plant Science and Resources, Okayama University, Chuo 2-10-1, 
      Kurashiki, Okayama 710-0046, Japan. Electronic address: 
      john.christeller@plantandfood.co.nz.
FAU - Galis, Ivan
AU  - Galis I
AD  - Institute of Plant Science and Resources, Okayama University, Chuo 2-10-1, 
      Kurashiki, Okayama 710-0046, Japan. Electronic address: 
      igalis@rib.okayama-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140804
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 0 (Oxylipins)
RN  - 0RBV727H71 (alpha-Linolenic Acid)
SB  - IM
MH  - Oryza/*metabolism
MH  - Oxylipins/*metabolism
MH  - Plant Leaves/*metabolism
MH  - alpha-Linolenic Acid/*metabolism
OTO - NOTNLM
OT  - 12-oxo-phytodienoic acid
OT  - Jasmonic acid
OT  - Octadecanoid pathway
OT  - Oryza sativa
OT  - alpha-Linolenic acid
EDAT- 2014/08/19 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/08/18 06:00
PHST- 2014/05/03 00:00 [received]
PHST- 2014/07/17 00:00 [accepted]
PHST- 2014/08/18 06:00 [entrez]
PHST- 2014/08/19 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0981-9428(14)00233-2 [pii]
AID - 10.1016/j.plaphy.2014.07.013 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2014 Oct;83:117-25. doi: 10.1016/j.plaphy.2014.07.013. 
      Epub 2014 Aug 4.