PMID- 25129578
OWN - NLM
STAT- MEDLINE
DCOM- 20150623
LR  - 20171116
IS  - 1572-9699 (Electronic)
IS  - 0003-6072 (Linking)
VI  - 106
IP  - 5
DP  - 2014 Nov
TI  - Bacterial diversity assessment in soil of an active Brazilian copper mine using 
      high-throughput sequencing of 16S rDNA amplicons.
PG  - 879-90
LID - 10.1007/s10482-014-0257-6 [doi]
AB  - Mining activities pose severe environmental risks worldwide, generating extreme 
      pH conditions and high concentrations of heavy metals, which can have major 
      impacts on the survival of organisms. In this work, pyrosequencing of the V3 
      region of the 16S rDNA was used to analyze the bacterial communities in soil 
      samples from a Brazilian copper mine. For the analysis, soil samples were 
      collected from the slopes (geotechnical structures) and the surrounding drainage 
      of the Sossego mine (comprising the Sossego and Sequeirinho deposits). The 
      results revealed complex bacterial diversity, and there was no influence of 
      deposit geographic location on the composition of the communities. However, the 
      environment type played an important role in bacterial community divergence; the 
      composition and frequency of OTUs in the slope samples were different from those 
      of the surrounding drainage samples, and Acidobacteria, Chloroflexi, Firmicutes, 
      and Gammaproteobacteria were responsible for the observed difference. Chemical 
      analysis indicated that both types of sample presented a high metal content, 
      while the amounts of organic matter and water were higher in the surrounding 
      drainage samples. Non-metric multidimensional scaling (N-MDS) analysis identified 
      organic matter and water as important distinguishing factors between the 
      bacterial communities from the two types of mine environment. Although 
      habitat-specific OTUs were found in both environments, they were more abundant in 
      the surrounding drainage samples (around 50 %), and contributed to the higher 
      bacterial diversity found in this habitat. The slope samples were dominated by a 
      smaller number of phyla, especially Firmicutes. The bacterial communities from 
      the slope and surrounding drainage samples were different in structure and 
      composition, and the organic matter and water present in these environments 
      contributed to the observed differences.
FAU - Rodrigues, Viviane D
AU  - Rodrigues VD
AD  - Center for Molecular Biology and Genetic Engineering (CBMEG), State University of 
      Campinas-UNICAMP, POB 6010, Campinas, SP, 13083-875, Brazil, 
      vivibiotec@gmail.com.
FAU - Torres, Tatiana T
AU  - Torres TT
FAU - Ottoboni, Laura M M
AU  - Ottoboni LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140817
PL  - Netherlands
TA  - Antonie Van Leeuwenhoek
JT  - Antonie van Leeuwenhoek
JID - 0372625
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Metals)
RN  - 0 (Organic Chemicals)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - 059QF0KO0R (Water)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Bacteria/*classification/*genetics
MH  - *Biota
MH  - Brazil
MH  - Cluster Analysis
MH  - Copper
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Metals/analysis
MH  - Organic Chemicals/analysis
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Soil/chemistry
MH  - *Soil Microbiology
MH  - Water/analysis
EDAT- 2014/08/19 06:00
MHDA- 2015/06/24 06:00
CRDT- 2014/08/18 06:00
PHST- 2014/02/07 00:00 [received]
PHST- 2014/08/08 00:00 [accepted]
PHST- 2014/08/18 06:00 [entrez]
PHST- 2014/08/19 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1007/s10482-014-0257-6 [doi]
PST - ppublish
SO  - Antonie Van Leeuwenhoek. 2014 Nov;106(5):879-90. doi: 10.1007/s10482-014-0257-6. 
      Epub 2014 Aug 17.