PMID- 25130664 OWN - NLM STAT- MEDLINE DCOM- 20150605 LR - 20161125 IS - 1872-6240 (Electronic) IS - 0006-8993 (Linking) VI - 1583 DP - 2014 Oct 2 TI - Kisspeptin-10 potentiates miniature excitatory postsynaptic currents in the rat supraoptic nucleus. PG - 45-54 LID - S0006-8993(14)01084-1 [pii] LID - 10.1016/j.brainres.2014.08.020 [doi] AB - Kisspeptin is the natural ligand of the G protein-coupled receptor -54 and plays a major role in gonadotropin-releasing hormone secretion in the hypothalamus. Kisspeptin-10 is an endogenous derivative of kisspeptin and has 10 -amino acids. Previous studies have demonstrated that central administration of kisspeptin-10 stimulates the secretion of arginine vasopressin (AVP) in male rats. We examined the effects of kisspeptin-10 on- excitatory synaptic inputs to magnocellular neurosecretory cells (MNCs) including AVP neurons in the supraoptic nucleus (SON) by obtaining in vitro whole-cell patch-clamp recordings from slice preparations of the rat brain. The application of kisspeptin-10 (100 nM-1 muM) significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) in a dose-related manner without affecting the amplitude. The kisspeptin-10-induced potentiation of the mEPSCs was significantly attenuated by previous exposure to the kisspeptin receptor antagonist kisspeptin-234 (100 nM) and to the protein kinase C inhibitor bisindolylmaleimide I (20 nM). These results suggest that kisspeptin-10 participates in the regulation of synaptic inputs to the MNCs in the SON by interacting with the kisspeptin receptor. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Yokoyama, Toru AU - Yokoyama T AD - Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan; Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan. FAU - Minami, Kouichiro AU - Minami K AD - Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan; Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan. FAU - Terawaki, Kiyoshi AU - Terawaki K AD - Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Tsumura Research Laboratories, Tsumura and Company, 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan. FAU - Miyano, Kanako AU - Miyano K AD - Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. FAU - Ogata, Junichi AU - Ogata J AD - Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan. FAU - Maruyama, Takashi AU - Maruyama T AD - Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. FAU - Takeuchi, Mamoru AU - Takeuchi M AD - Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan. FAU - Uezono, Yasuhito AU - Uezono Y AD - Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. FAU - Ueta, Yoichi AU - Ueta Y AD - Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. Electronic address: yoichi@med.uoeh-u.ac.jp. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140815 PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Enzyme Inhibitors) RN - 0 (Indoles) RN - 0 (Kiss1 protein, rat) RN - 0 (Kisspeptins) RN - 0 (Maleimides) RN - 0 (enhanced green fluorescent protein) RN - 113-79-1 (Arginine Vasopressin) RN - 147336-22-9 (Green Fluorescent Proteins) RN - EC 2.7.11.13 (Protein Kinase C) RN - L79H6N0V6C (bisindolylmaleimide I) SB - IM MH - Animals MH - Arginine Vasopressin/genetics/metabolism MH - Enzyme Inhibitors/pharmacology MH - Excitatory Postsynaptic Potentials/drug effects/*physiology MH - Green Fluorescent Proteins/genetics/metabolism MH - Indoles/pharmacology MH - Inhibitory Postsynaptic Potentials/drug effects/physiology MH - Kisspeptins/*metabolism MH - Male MH - Maleimides/pharmacology MH - Miniature Postsynaptic Potentials/drug effects/*physiology MH - Neurons/drug effects/*physiology MH - Patch-Clamp Techniques MH - Protein Kinase C/antagonists & inhibitors/metabolism MH - Rats, Transgenic MH - Rats, Wistar MH - Supraoptic Nucleus/drug effects/*physiology MH - Tissue Culture Techniques OTO - NOTNLM OT - EPSC OT - IPSC OT - Kisspeptin OT - Slice patch clamp OT - Supraoptic nucleus EDAT- 2014/08/19 06:00 MHDA- 2015/06/06 06:00 CRDT- 2014/08/19 06:00 PHST- 2014/03/14 00:00 [received] PHST- 2014/08/06 00:00 [revised] PHST- 2014/08/08 00:00 [accepted] PHST- 2014/08/19 06:00 [entrez] PHST- 2014/08/19 06:00 [pubmed] PHST- 2015/06/06 06:00 [medline] AID - S0006-8993(14)01084-1 [pii] AID - 10.1016/j.brainres.2014.08.020 [doi] PST - ppublish SO - Brain Res. 2014 Oct 2;1583:45-54. doi: 10.1016/j.brainres.2014.08.020. Epub 2014 Aug 15.