PMID- 25132285
OWN - NLM
STAT- MEDLINE
DCOM- 20161018
LR  - 20211021
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 29
IP  - 6
DP  - 2015 Nov
TI  - Comparison of Chromogenic In Situ Hybridization and Fluorescence In Situ 
      Hybridization for the Evaluation of MDM2 Amplification in Adipocytic Tumors.
PG  - 462-8
LID - 10.1002/jcla.21794 [doi]
AB  - BACKGROUND: Atypical lipomatous tumor/well-differentiated liposarcoma (ALT-WDLPS) 
      and dedifferentiated liposarcoma (DDLPS) are characterized cytogenetically by a 
      12q13-15 amplification involving the mouse double minute 2 (MDM2) oncogene. 
      Fluorescence in situ hybridization (FISH) is used frequently to detect this 
      amplification and aid with the diagnosis of these entities, which is difficult by 
      morphology alone. Recently, bright-field in situ hybridization techniques such as 
      chromogenic in situ hybridization (CISH) have been introduced for the 
      determination of MDM2 amplification status. METHODS: The present study compared 
      the results of FISH and CISH for detecting MDM2 amplification in 41 cases of 
      adipocytic tumors. Amplification was defined in both techniques as a MDM2/CEN12 
      ratio of 2 or greater. RESULTS: Eleven cases showed amplification with both FISH 
      and CISH, and 26 cases showed no amplification with both methods. Two cases had 
      discordant results between CISH and FISH, and two cases were not interpretable by 
      CISH. CONCLUSION: CISH is advantageous for allowing pathologists to evaluate the 
      histologic and molecular alterations occurring simultaneously in a specimen. 
      Moreover, CISH is found to be more cost- and time-efficient when used with 
      automation, and the signals do not quench over time. CISH technique is a reliable 
      alternative to FISH in the evaluation of adipocytic tumors for MDM2 
      amplification.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Mardekian, Stacey K
AU  - Mardekian SK
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
FAU - Solomides, Charalambos C
AU  - Solomides CC
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
FAU - Gong, Jerald Z
AU  - Gong JZ
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
FAU - Peiper, Stephen C
AU  - Peiper SC
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
FAU - Wang, Zi-Xuan
AU  - Wang ZX
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
FAU - Bajaj, Renu
AU  - Bajaj R
AD  - Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140817
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Adipocytes/metabolism/*pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Biomarkers, Tumor/*genetics
MH  - Case-Control Studies
MH  - Female
MH  - Follow-Up Studies
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization/*classification/*methods
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-mdm2/*genetics
MH  - Soft Tissue Neoplasms/*genetics/pathology
PMC - PMC6806684
OTO - NOTNLM
OT  - MDM2 protein
OT  - adipose tissue
OT  - human gene amplification in situ hybridization chromogenic compounds
OT  - neoplasms
COIS- Ventana Medical Systems, Inc. (Tucson, Arizona) provided our institution 
      free-of-charge with the necessary probes and reagents to carry out the CISH 
      portion of the study.
EDAT- 2014/08/19 06:00
MHDA- 2016/10/19 06:00
PMCR- 2014/08/17
CRDT- 2014/08/19 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/06/12 00:00 [accepted]
PHST- 2014/08/19 06:00 [entrez]
PHST- 2014/08/19 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
PHST- 2014/08/17 00:00 [pmc-release]
AID - JCLA21794 [pii]
AID - 10.1002/jcla.21794 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2015 Nov;29(6):462-8. doi: 10.1002/jcla.21794. Epub 2014 Aug 17.