PMID- 25136126
OWN - NLM
STAT- MEDLINE
DCOM- 20141112
LR  - 20220309
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 111
IP  - 35
DP  - 2014 Sep 2
TI  - A unique PDZ domain and arrestin-like fold interaction reveals mechanistic 
      details of endocytic recycling by SNX27-retromer.
PG  - E3604-13
LID - 10.1073/pnas.1410552111 [doi]
AB  - The sorting nexin 27 (SNX27)-retromer complex is a major regulator of 
      endosome-to-plasma membrane recycling of transmembrane cargos that contain a 
      PSD95, Dlg1, zo-1 (PDZ)-binding motif. Here we describe the core interaction in 
      SNX27-retromer assembly and its functional relevance for cargo sorting. Crystal 
      structures and NMR experiments reveal that an exposed beta-hairpin in the SNX27 PDZ 
      domain engages a groove in the arrestin-like structure of the vacuolar protein 
      sorting 26A (VPS26A) retromer subunit. The structure establishes how the SNX27 
      PDZ domain simultaneously binds PDZ-binding motifs and retromer-associated VPS26. 
      Importantly, VPS26A binding increases the affinity of the SNX27 PDZ domain for 
      PDZ- binding motifs by an order of magnitude, revealing cooperativity in cargo 
      selection. With disruption of SNX27 and retromer function linked to synaptic 
      dysfunction and neurodegenerative disease, our work provides the first step, to 
      our knowledge, in the molecular description of this important sorting complex, 
      and more broadly describes a unique interaction between a PDZ domain and an 
      arrestin-like fold.
FAU - Gallon, Matthew
AU  - Gallon M
AD  - The Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, 
      University of Bristol, Bristol BS8 1TD, United Kingdom;
FAU - Clairfeuille, Thomas
AU  - Clairfeuille T
AD  - Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 
      4072, Australia; and.
FAU - Steinberg, Florian
AU  - Steinberg F
AD  - The Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, 
      University of Bristol, Bristol BS8 1TD, United Kingdom;
FAU - Mas, Caroline
AU  - Mas C
AD  - Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 
      4072, Australia; and.
FAU - Ghai, Rajesh
AU  - Ghai R
AD  - Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 
      4072, Australia; and.
FAU - Sessions, Richard B
AU  - Sessions RB
AD  - School of Biochemistry, University of Bristol, Bristol BS8 1TD, United Kingdom.
FAU - Teasdale, Rohan D
AU  - Teasdale RD
AD  - Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 
      4072, Australia; and.
FAU - Collins, Brett M
AU  - Collins BM
AD  - Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 
      4072, Australia; and b.collins@imb.uq.edu.au pete.cullen@bristol.ac.uk.
FAU - Cullen, Peter J
AU  - Cullen PJ
AD  - The Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, 
      University of Bristol, Bristol BS8 1TD, United Kingdom; b.collins@imb.uq.edu.au 
      pete.cullen@bristol.ac.uk.
LA  - eng
SI  - PDB/4P2A
GR  - MR/K018299/1/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140818
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Arrestin)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SNX27 protein, human)
RN  - 0 (Snx27 protein, mouse)
RN  - 0 (Snx27 protein, rat)
RN  - 0 (Sorting Nexins)
RN  - 0 (VPS26A protein, human)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (Vps26 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arrestin/chemistry/genetics
MH  - Brain Diseases/genetics/metabolism/pathology
MH  - Crystallography, X-Ray
MH  - Endocytosis/*physiology
MH  - Endosomes/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Nerve Tissue Proteins/chemistry/genetics/metabolism
MH  - PDZ Domains/*genetics
MH  - Protein Folding
MH  - Protein Sorting Signals/genetics
MH  - RNA, Small Interfering/genetics
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Sorting Nexins/*chemistry/genetics/metabolism
MH  - Vesicular Transport Proteins/*chemistry/genetics/metabolism
PMC - PMC4156734
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Down syndrome
OT  - Parkinson disease
OT  - endosomal recycling
COIS- The authors declare no conflict of interest.
EDAT- 2014/08/20 06:00
MHDA- 2014/11/13 06:00
PMCR- 2015/03/02
CRDT- 2014/08/20 06:00
PHST- 2014/08/20 06:00 [entrez]
PHST- 2014/08/20 06:00 [pubmed]
PHST- 2014/11/13 06:00 [medline]
PHST- 2015/03/02 00:00 [pmc-release]
AID - 1410552111 [pii]
AID - 201410552 [pii]
AID - 10.1073/pnas.1410552111 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):E3604-13. doi: 
      10.1073/pnas.1410552111. Epub 2014 Aug 18.