PMID- 2514540
OWN - NLM
STAT- MEDLINE
DCOM- 19900214
LR  - 20141120
IS  - 0237-6261 (Print)
IS  - 0237-6261 (Linking)
VI  - 24
IP  - 1-2
DP  - 1989
TI  - Volume regulatory mechanisms of human granulocytes in hypoosmotic media.
PG  - 83-99
AB  - Human neutrophil granulocytes, when placed into hypoosmotic media, first swell, 
      than show a regulatory volume decrease (RVD) reaction. This RVD is the most 
      effective at pH 7.5 when inosine is used as a metabolic substrate. The RVD 
      reaction is blocked by the inhibitors of the calcium-induced K+ transport, such 
      as quinine, quinidine, trifluoperazine (TFP), as well as by those of the 
      conductive Cl-transport (dipyridamole, SITS, oligomycin C). Removal of external 
      calcium by EGTA, or addition of the calcium channel inhibitor, verapamil, reduce 
      the RVD. Ouabain affects RVD only after long preincubation and furosemide or 
      phloretin have hardly any effect on this process. In a hypoosmotic KCl medium 
      neutrophils show a TFP-, and SITS-sensitive secondary volume increase. All these 
      data indicate the opening of Ca2+-induced K+ channels and of conductive Cl- 
      channels in hypoosmotically shocked neutrophils. Under these conditions, however, 
      direct measurement of cytoplasmic calcium by Indo-1 does not show any major 
      change in the overall cytoplasmic free Ca2+ levels. In neutrophils, the reduced 
      calcium signal evoked by formyl-methionyl-leucyl-phenylalanine (fMLP) after a 
      hypoosmotic shock, suggests that cellular calcium metabolism is altered under 
      these conditions.
FAU - Kovacs, T
AU  - Kovacs T
AD  - National Institute of Haematology and Blood Transfusion, Hungary.
FAU - Szasz, I
AU  - Szasz I
FAU - Sarkadi, B
AU  - Sarkadi B
FAU - Brezanoczy, F
AU  - Brezanoczy F
FAU - Gardos, G
AU  - Gardos G
LA  - eng
PT  - Journal Article
PL  - Hungary
TA  - Acta Biochim Biophys Hung
JT  - Acta biochimica et biophysica Hungarica
JID - 8701158
RN  - 0 (Chlorides)
RN  - 0 (Potassium Channels)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Biological Transport, Active
MH  - Calcium/metabolism
MH  - Chlorides/metabolism
MH  - Granulocytes/cytology/drug effects/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/metabolism
MH  - Osmotic Pressure
MH  - Potassium/metabolism
MH  - Potassium Channels/metabolism
MH  - Water-Electrolyte Balance
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
PST - ppublish
SO  - Acta Biochim Biophys Hung. 1989;24(1-2):83-99.