PMID- 25146601
OWN - NLM
STAT- MEDLINE
DCOM- 20150110
LR  - 20211021
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 464-465
DP  - 2014 Sep
TI  - The interaction of nonstructural protein 9 with retinoblastoma protein benefits 
      the replication of genotype 2 porcine reproductive and respiratory syndrome virus 
      in vitro.
PG  - 432-440
LID - S0042-6822(14)00351-1 [pii]
LID - 10.1016/j.virol.2014.07.036 [doi]
AB  - The nonstructural protein 9 (Nsp9) of porcine reproductive and respiratory 
      syndrome virus (PRRSV) is a RNA-dependent RNA polymerase (RdRp) that plays a 
      vital role in viral replication. This study first demonstrated that the Nsp9 of 
      genotype 2 PRRSV interacted with cellular retinoblastoma protein (pRb), and Nsp9 
      co-localized with pRb in the cytoplasm of PRRSV-infected MARC-145 cells and 
      pulmonary alveolar macrophages (PAMs). Next, the overexpression of truncated pRb 
      was shown to inhibit the PRRSV replication and silencing the pRb gene could 
      facilitate the PRRSV replication in MARC-145 cells. Finally, the pRb level was 
      confirmed to be down-regulated in PRRSV-infected MARC-145 cells, and Nsp9 was 
      shown to promote the pRb degradation by proteasome pathway. These findings 
      indicate that the interaction of Nsp 9 with pRb benefits the replication of 
      genotype 2 PRRSV in vitro, helping to understand the roles of Nsp9 in the 
      replication and pathogenesis of PRRSV.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Dong, Jianguo
AU  - Dong J
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China. Electronic address: guoxin@cau.edu.cn.
FAU - Yang, Hanchun
AU  - Yang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of 
      Agriculture, College of Veterinary Medicine and State Key Laboratory of 
      Agrobiotechnology, China Agricultural University, Beijing, People's Republic of 
      China. Electronic address: yanghanchun1@cau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140821
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Down-Regulation
MH  - Genotype
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/genetics/*physiology
MH  - Proteasome Endopeptidase Complex/genetics/metabolism
MH  - Protein Binding
MH  - Proteolysis
MH  - Retinoblastoma Protein/genetics/*metabolism
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - *Virus Replication
PMC - PMC7112046
OTO - NOTNLM
OT  - Genotype 2
OT  - Interaction
OT  - Nonstructural protein 9 (Nsp9)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Replication
OT  - Retinoblastoma protein (pRb)
EDAT- 2014/08/26 06:00
MHDA- 2015/01/13 06:00
PMCR- 2014/08/21
CRDT- 2014/08/23 06:00
PHST- 2014/04/23 00:00 [received]
PHST- 2014/07/18 00:00 [revised]
PHST- 2014/07/21 00:00 [accepted]
PHST- 2014/08/23 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
PHST- 2014/08/21 00:00 [pmc-release]
AID - S0042-6822(14)00351-1 [pii]
AID - 10.1016/j.virol.2014.07.036 [doi]
PST - ppublish
SO  - Virology. 2014 Sep;464-465:432-440. doi: 10.1016/j.virol.2014.07.036. Epub 2014 
      Aug 21.