PMID- 25146741
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20171116
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 94
IP  - 20
DP  - 2014 May 27
TI  - [Level of soluble programmed death ligand 1 in pleural effusion and peripheral 
      blood of patients with tuberculous pleural effusion and its clinical 
      implications].
PG  - 1543-6
AB  - OBJECTIVE: To explore the level of soluble programmed death ligand 1 (sPD-L1) in 
      pleural effusion and peripheral blood of patients with tuberculous pleural 
      effusion (TPE) and elucidate its clinical implications. METHODS: Patients with 
      newly diagnosed pleural effusion at the Second Affiliated Hospital of Soochow 
      University from June 2012 to March 2013 were enrolled and divided into 3 groups 
      of TPE, malignant pleural effusion (MPE) and non-tuberculous non-malignant 
      pleural effusion (non-TPE non-MPE) according to the nature of pleural effusion. 
      The level of sPD-L1 in pleural effusion and peripheral blood was analyzed by 
      enzyme linked immunosorbent assay (ELISA) kit. Flow cytometry was used to detect 
      the changes of immune cell subsets in pleural effusion. And the gene expressions 
      of programmed death ligand 1 (PD-L1) and matrix metalloproteinase-3 (MMP-3) were 
      detected in different effusions by reverse transcription-polymerase chain 
      reaction (RT-PCR). RESULTS: A total of 77 newly diagnosed patients with pleural 
      effusion were enrolled, 24 patients with TPE, 39 patients with MPE, 14 patients 
      with non-TPE non-MPE. The level of sPD-L1 in TPE was higher than that in MPE and 
      non-TPE non-MPE (4.2 (2.6-6.3), 1.4 (0.8-2.1), 1.8 (1.2-2.6) microg/L, P < 0.001). No 
      significant difference existed in the levels of sPD-L1 in peripheral blood 
      samples (P = 0.811). The average content of sPD-L1 in pleural effusion in all 
      patients was statistically higher than that in peripheral blood (2.0 (1.4-3.7), 
      1.5 (1.0-2.0) microg/L, P = 0.004). The proportion of CD8 subset, PD-L1 on CD14(+) 
      monocytes and the mRNA level of PD-L1, MMP-3 in TPE were higher than in MPE and 
      non-TPE non-MPE (P = 0.001, P < 0.001, P < 0.001), and the mRNA level of PD-L1 in 
      TPE was positively correlated with the level of MMP-3 (r = 0.887, P < 0.001). 
      Receiver operating characteristic (ROC) curve analysis showed that sPD-L1 had a 
      sensitivity of 82.6%, a specificity of 83.8% and an area under curve (AUC) of 
      0.854 for differential diagnosis of TPE from other conditions. Combinations of 
      sPD-L1, PD-L1 on CD14(+) monocytes and adenosine deaminase (ADA) measurements 
      further increased the sensitivity up to 91.3%, specificity up to 89.2% and AUC up 
      to 0.989. CONCLUSION: The elevated expression of sPD-L1 in tuberculous pleural 
      effusion may aid the diagnosis of TPE.
FAU - Pan, Xue
AU  - Pan X
AD  - Department of Respiratory Medicine, Second Affiliated Hospital, Soochow 
      University, Suzhou 215004, China.
FAU - Xing, Yufei
AU  - Xing Y
FAU - Shi, Minhua
AU  - Shi M
AD  - Email: shiminhua@163.com.
FAU - Zhou, Tong
AU  - Zhou T
FAU - Qian, Bin
AU  - Qian B
FAU - Chen, Yongjing
AU  - Chen Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (B7-H1 Antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B7-H1 Antigen/blood/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pleural Effusion/metabolism
MH  - Tuberculosis, Pleural/*diagnosis/immunology/metabolism
MH  - Young Adult
EDAT- 2014/08/26 06:00
MHDA- 2015/01/30 06:00
CRDT- 2014/08/23 06:00
PHST- 2014/08/23 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2014 May 27;94(20):1543-6.