PMID- 25148576
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20220310
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 139
IP  - 21
DP  - 2014 Nov 7
TI  - Magnetic solid phase extraction coupled with in situ derivatization for the 
      highly sensitive determination of acidic phytohormones in rice leaves by 
      UPLC-MS/MS.
PG  - 5605-13
LID - 10.1039/c4an01186d [doi]
AB  - A simple, rapid and sensitive method based on magnetic solid phase extraction 
      (MSPE) coupled with in situ derivatization (ISD) was developed for the 
      determination of endogenous acidic phytohormones in rice leaves by 
      ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) 
      analysis. With this method, acidic phytohormones were extracted onto the surface 
      of a TiO2/magnetic hollow mesoporous silica sphere (MHMSS) through hydrophilic 
      interaction, and then in situ derivatization was performed by the subsequent 
      addition of 3-bromoactonyltrimethylammonium bromide (BTA). Thus, the process 
      integrated extraction, purification, and derivatization into one step. 
      Additionally, the permanent positively charged moiety from BTA significantly 
      improved the ionization efficiencies of the acidic phytohormones. Several 
      parameters affecting the efficiencies of the extraction, derivatization, and 
      desorption were evaluated. The signal intensities of acidic phytohormones 
      increased by 2 to 481 fold after treatment with MSPE-ISD. Under the optimized 
      conditions, several endogenous acidic phytohormones, including GA4, GA9, GA20, 
      JA, IAA, and ABA, were identified and quantified in rice leaves by the MSPE-ISD 
      method. The limits of detection (LODs) were in the range of 1.03-91.21 pg mL(-1). 
      The relative recoveries ranged from 71.6-112.8%, with the intra- and interday 
      relative standard deviations (RSDs) being less than 14.9% and 16.2%, 
      respectively. Taken together, the proposed method provides a novel approach of 
      combining magnetic solid phase extraction and in situ derivatization for the 
      highly sensitive determination of endogenous acidic phytohormones.
FAU - Liu, Jiu-Feng
AU  - Liu JF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China. 
      yqfeng@whu.edu.cn.
FAU - Ding, Jun
AU  - Ding J
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Acids)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Acids/chemistry
MH  - Chromatography, Liquid/*methods
MH  - Limit of Detection
MH  - *Magnetics
MH  - Oryza/*chemistry
MH  - Plant Growth Regulators/*analysis
MH  - Plant Leaves/*chemistry
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2014/08/26 06:00
MHDA- 2015/08/25 06:00
CRDT- 2014/08/23 06:00
PHST- 2014/08/23 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - 10.1039/c4an01186d [doi]
PST - ppublish
SO  - Analyst. 2014 Nov 7;139(21):5605-13. doi: 10.1039/c4an01186d.