PMID- 25149524
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20211021
IS  - 1532-2807 (Electronic)
IS  - 1219-4956 (Linking)
VI  - 21
IP  - 2
DP  - 2015 Apr
TI  - Mutational Status of CDKN2A and TP53 Genes in Laryngeal Squamous Cell Carcinoma.
PG  - 413-21
LID - 10.1007/s12253-014-9836-0 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is the second most common tumour of the 
      head and neck. It is characterized by frequent aberrations in two cell-cycle 
      regulators--CDKN2A and TP53. However, LSCC has been often studied as a part of 
      the group of head and neck cancers and not as an individual entity. In the 
      current study we aimed to examine mutation status of CDKN2A and TP53 genes in 108 
      LSCC patients. DNA was extracted from fresh-frozen tumour tissues; exons 1-3 of 
      CDKN2A and exons 5-8 of TP53 were screened for mutations by direct sequencing. 
      Genetic aberrations in CDKN2A were found in 16 (14.2%) and those in TP53--in 
      56/108 (51.9%) tumours. Seven mutations (two insertions, three deletions, one 
      missense and one silent) detected in CDKN2A were not described previously. Also, 
      we found seven novel deletions and a novel indel in TP53. No significant 
      associations with clinical features were found. However, TP53 mutations were 
      predominantly observed in smokers with advanced stage tumours. Screening for 
      genetic aberrations in a defined group of LSCC contributes to the knowledge about 
      laryngeal carcinogenesis. Further investigations are required to confirm the 
      observed trends in associations with clinical features.
FAU - Todorova, Teodora A
AU  - Todorova TA
AD  - Molecular Medicine Center, Medical University-Sofia, 2 Zdrave street, Sofia, 
      1431, Bulgaria.
FAU - Jordanov, Stanislav H
AU  - Jordanov SH
FAU - Stancheva, Gergana S
AU  - Stancheva GS
FAU - Chalakov, Ivan J
AU  - Chalakov IJ
FAU - Melnicharov, Mincho B
AU  - Melnicharov MB
FAU - Kunev, Kuncho V
AU  - Kunev KV
FAU - Mitev, Vanio I
AU  - Mitev VI
FAU - Kaneva, Radka P
AU  - Kaneva RP
FAU - Goranova, Teodora E
AU  - Goranova TE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140823
PL  - Switzerland
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Genes, Neoplasm/genetics
MH  - Head and Neck Neoplasms/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Mutation/*genetics
MH  - Prognosis
MH  - Sequence Analysis, DNA
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2014/08/26 06:00
MHDA- 2016/02/24 06:00
CRDT- 2014/08/24 06:00
PHST- 2014/05/24 00:00 [received]
PHST- 2014/08/13 00:00 [accepted]
PHST- 2014/08/24 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
AID - 10.1007/s12253-014-9836-0 [doi]
PST - ppublish
SO  - Pathol Oncol Res. 2015 Apr;21(2):413-21. doi: 10.1007/s12253-014-9836-0. Epub 
      2014 Aug 23.