PMID- 25150168
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20161125
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 588
IP  - 20
DP  - 2014 Oct 16
TI  - Expression of type I collagen and tenascin C is regulated by actin polymerization 
      through MRTF in dedifferentiated chondrocytes.
PG  - 3677-84
LID - S0014-5793(14)00613-9 [pii]
LID - 10.1016/j.febslet.2014.08.012 [doi]
AB  - This study examined actin regulation of fibroblast matrix genes in 
      dedifferentiated chondrocytes. We demonstrated that dedifferentiated chondrocytes 
      exhibit increased actin polymerization, nuclear localization of myocardin related 
      transcription factor (MRTF), increased type I collagen (col1) and tenascin C 
      (Tnc) gene expression, and decreased Sox9 gene expression. Induction of actin 
      depolymerization by latrunculin treatment or cell rounding, reduced MRTF nuclear 
      localization, repressed col1 and Tnc expression, and increased Sox9 gene 
      expression in dedifferentiated chondrocytes. Treatment of passaged chondrocytes 
      with MRTF inhibitor repressed col1 and Tnc expression, but did not affect Sox9 
      expression. Our results show that actin polymerization regulates fibroblast 
      matrix gene expression through MRTF in passaged chondrocytes.
CI  - Copyright (c) 2014 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Parreno, Justin
AU  - Parreno J
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada; Department of Pathology and Laboratory Medicine, Mount 
      Sinai Hospital, Toronto, Canada.
FAU - Raju, Sneha
AU  - Raju S
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada.
FAU - Niaki, Mortah Nabavi
AU  - Niaki MN
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada; Institute of Biomaterials and Biomedical Engineering, 
      University of Toronto, Canada.
FAU - Andrejevic, Katarina
AU  - Andrejevic K
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada; Department of Pathology and Laboratory Medicine, Mount 
      Sinai Hospital, Toronto, Canada.
FAU - Jiang, Amy
AU  - Jiang A
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada.
FAU - Delve, Elizabeth
AU  - Delve E
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada; Institute of Biomaterials and Biomedical Engineering, 
      University of Toronto, Canada.
FAU - Kandel, Rita
AU  - Kandel R
AD  - CIHR-BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of 
      Toronto, Toronto, Canada; Lunenfeld-Tanenbaum Research Institute, Mount Sinai 
      Hospital, Toronto, Canada; Department of Laboratory Medicine and Pathobiology, 
      University of Toronto, Canada; Department of Pathology and Laboratory Medicine, 
      Mount Sinai Hospital, Toronto, Canada; Institute of Biomaterials and Biomedical 
      Engineering, University of Toronto, Canada. Electronic address: 
      rkandel@mtsinai.on.ca.
LA  - eng
GR  - MOP126111/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140819
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Actins)
RN  - 0 (Anilides)
RN  - 0 (Benzamides)
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (CCG 1423)
RN  - 0 (Collagen Type I)
RN  - 0 (Tenascin)
RN  - 0 (Thiazolidines)
RN  - 0 (Transcription Factors)
RN  - LW7U308U7U (latrunculin B)
SB  - IM
MH  - Actins/*metabolism
MH  - Active Transport, Cell Nucleus
MH  - Anilides/pharmacology
MH  - Animals
MH  - Benzamides/pharmacology
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Cattle
MH  - Cell Dedifferentiation
MH  - Cell Nucleus/metabolism
MH  - Chondrocytes/cytology/drug effects/*metabolism
MH  - Collagen Type I/genetics/*metabolism
MH  - Polymerization
MH  - Tenascin/genetics/*metabolism
MH  - Thiazolidines/pharmacology
MH  - Transcription Factors/*metabolism
OTO - NOTNLM
OT  - Actin
OT  - Chondrocyte
OT  - Collagen
OT  - Dedifferentiation
OT  - Myocardin related transcription factor
OT  - Tenascin C
EDAT- 2014/08/26 06:00
MHDA- 2014/11/19 06:00
CRDT- 2014/08/24 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/08/05 00:00 [revised]
PHST- 2014/08/11 00:00 [accepted]
PHST- 2014/08/24 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
AID - S0014-5793(14)00613-9 [pii]
AID - 10.1016/j.febslet.2014.08.012 [doi]
PST - ppublish
SO  - FEBS Lett. 2014 Oct 16;588(20):3677-84. doi: 10.1016/j.febslet.2014.08.012. Epub 
      2014 Aug 19.