PMID- 25152053
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20220623
IS  - 0015-5500 (Print)
IS  - 0015-5500 (Linking)
VI  - 60
IP  - 4
DP  - 2014
TI  - Complementarity between microarray and immunoblot for the comparative evaluation 
      of IgE repertoire of French and Italian cypress pollen allergic patients.
PG  - 192-201
AB  - Cypress pollen represents the primary cause of respiratory allergies in 
      Mediterranean areas. Patients allergic to Cupressus sempervirens pollen (Cups) 
      (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to 
      a basic 14 kDa protein (BP14) or to high-molecular-weight (HMW) glycoproteins 
      only. Specific IgE repertoires of two differentially exposed CPA cohorts, French 
      and Italian, were investigated using an IgE microarray system (some known major 
      allergens from several allergenic sources) and individual IgE immunoblotting (IB) 
      of whole Cups pollen extract separated by SDS-PAGE (all allergens from one 
      allergenic source: cypress pollen). The prevalence of sensitization to BP14 was 
      higher in French (37 %) than in Italian patients (17 %) and major differences 
      were observed in IgE reactivities to lipid transfer proteins (LTPs). Thirty 
      percent of the Italian CPA (4 % in the French group) had specific IgE against the 
      Parietaria pollen LTP, independently of IB subgroups. Regarding peach LTP 
      sensitization, all Pru p 3+ Italian CPA (10 %) were in the HMW+ subgroup, while 
      Pru p 3+ French CPA (20 %) were all included in the BP14+ subgroup. BP14 
      sensitization is likely a marker of Cups exposure and is, in French CPA, 
      significantly correlated to Pru p 3 sensitization. The IgE immunoblot and 
      microarray are complementary tools that highlight differences in the subtle 
      sensitization profile between groups of patients in comparative studies.
FAU - Shahali, Y
AU  - Shahali Y
AD  - Armand Trousseau Hospital, Biochemistry and Molecular Biology D epartment, 
      Allergy & Environment Group, Paris, France.
FAU - Nicaise, P
AU  - Nicaise P
AD  - Bichat-Claude Bernard Hospital, Auto-immunity & Hypersensitivity D epartment, 
      Paris, France.
FAU - Brazdova, A
AU  - Brazdova A
AD  - Institute of Chemical Technology, Department of Biochemistry and Microbiology, 
      Prague, Czech Republic.
FAU - Charpin, D
AU  - Charpin D
AD  - North Hospital, Pneumo-Allergology D epartment, Marseille Cedex 20, France.
FAU - Scala, E
AU  - Scala E
AD  - IDI-IRCCS, Experimental Allergology D epartment, Rome, Italy.
FAU - Mari, A
AU  - Mari A
AD  - CAAM, Latina, Italy.
FAU - Sutra, J P
AU  - Sutra JP
AD  - Armand Trousseau Hospital, Biochemistry and Molecular Biology D epartment, 
      Allergy & Environment Group, Paris, France.
FAU - Chollet-Martin, S
AU  - Chollet-Martin S
AD  - Bichat-Claude Bernard Hospital, Auto-immunity & Hypersensitivity D epartment, 
      Paris, France.
FAU - Senechal, H
AU  - Senechal H
AD  - Armand Trousseau Hospital, Biochemistry and Molecular Biology D epartment, 
      Allergy & Environment Group, Paris, France.
FAU - Poncet, P
AU  - Poncet P
AD  - Armand Trousseau Hospital, Biochemistry and Molecular Biology D epartment, 
      Allergy & Environment Group, Paris, France.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Czech Republic
TA  - Folia Biol (Praha)
JT  - Folia biologica
JID - 0234640
RN  - 0 (Allergens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (lipid transfer protein)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Allergens/*immunology
MH  - Carrier Proteins/immunology
MH  - Child
MH  - Cohort Studies
MH  - Cupressus/*chemistry
MH  - Female
MH  - France
MH  - Humans
MH  - Hypersensitivity/epidemiology/*immunology
MH  - Immunization
MH  - Immunoblotting/*methods
MH  - Immunoglobulin E/*immunology
MH  - Italy/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Plant Proteins/immunology
MH  - Pollen/*immunology
MH  - Prevalence
MH  - Young Adult
EDAT- 2014/08/26 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/08/26 06:00
PHST- 2014/08/26 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - file/5731/fb2014a0023.pdf [pii]
PST - ppublish
SO  - Folia Biol (Praha). 2014;60(4):192-201.