PMID- 25152701
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1550-7033 (Print)
IS  - 1550-7041 (Electronic)
IS  - 1550-7033 (Linking)
VI  - 4
IP  - 4
DP  - 2008 Dec 1
TI  - Development of Receptor Targeted Magnetic Iron Oxide Nanoparticles for Efficient 
      Drug Delivery and Tumor Imaging.
PG  - 439-449
AB  - The development of multifunctional nanoparticles that have dual capabilities of 
      tumor imaging and delivering therapeutic agents into tumor cells holds great 
      promises for novel approaches for tumor imaging and therapy. We have engineered 
      urokinase plasminogen activator receptor (uPAR) targeted biodegradable 
      nanoparticles using a size uniform and amphiphilic polymer-coated magnetic iron 
      oxide (IO) nanoparticle conjugated with the amino-terminal fragment (ATF) of 
      urokinase plasminogen activator (uPA), which is a high affinity natural ligand 
      for uPAR. We further developed methods to encapsulate hydrophobic 
      chemotherapeutic drugs into the polymer layer on the IO nanoparticles, making 
      these targeted magnetic resonance imaging (MRI) sensitive nanoparticles drug 
      delivery vehicles. Using a fluorescent drug doxorubicin (Dox) as a model system, 
      we showed that this hydrophobic drug can be efficiently encapsulated into the 
      uPAR-targeted IO nanoparticles. This class of Dox-loaded nanoparticles has a 
      compact size and is stable in pH 7.4 buffer. However, encapsulated Doxcan be 
      released from the nanoparticles at pH 4.0 to 5.0 within 2 hrs. In comparison with 
      the effect of equivalent dosage of free drug or non-targeted IO-Dox 
      nanoparticles, uPAR-targeted IO-Dox nanoparticles deliver higher levels of Dox 
      into breast cancer cells and produce a stronger inhibitory effect on tumor cell 
      growth. Importantly, Dox-loaded IO nanoparticles maintain their T2 MRI contrast 
      effect after being internalized into the tumor cells due to their significant 
      susceptibility effect in the cells, indicating that this drug delivery 
      nanoparticle has the potential to be used as targeted therapeutic imaging probes 
      for monitoring the drug delivery using MRI.
FAU - Yang, Lily
AU  - Yang L
AD  - Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA ; Radiology, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA.
FAU - Cao, Zehong
AU  - Cao Z
AD  - Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA.
FAU - Sajja, Hari Krishna
AU  - Sajja HK
AD  - Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA.
FAU - Mao, Hui
AU  - Mao H
AD  - Radiology, Emory University School of Medicine, Atlanta, Georgia, GA 30322, USA.
FAU - Wang, Liya
AU  - Wang L
AD  - Radiology, Emory University School of Medicine, Atlanta, Georgia, GA 30322, USA.
FAU - Geng, Huaying
AU  - Geng H
AD  - Ocean Nanotech, LLC, 700 Research Center Blvd, 72701, Fayetteville, Arkansas, 
      USA.
FAU - Xu, Hengyi
AU  - Xu H
AD  - Ocean Nanotech, LLC, 700 Research Center Blvd, 72701, Fayetteville, Arkansas, 
      USA.
FAU - Jiang, Tieshan
AU  - Jiang T
AD  - Ocean Nanotech, LLC, 700 Research Center Blvd, 72701, Fayetteville, Arkansas, 
      USA.
FAU - Wood, William C
AU  - Wood WC
AD  - Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA.
FAU - Nie, Shuming
AU  - Nie S
AD  - Biomedical Engineering, Emory University School of Medicine, Atlanta, Georgia, GA 
      30322, USA.
FAU - Wang, Y Andrew
AU  - Wang YA
AD  - Ocean Nanotech, LLC, 700 Research Center Blvd, 72701, Fayetteville, Arkansas, 
      USA.
LA  - eng
GR  - R01 CA163256/CA/NCI NIH HHS/United States
GR  - R01 CA133722/CA/NCI NIH HHS/United States
GR  - U54 CA119338/CA/NCI NIH HHS/United States
GR  - R01 CA133722-02/CA/NCI NIH HHS/United States
GR  - R01 CA154846/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
PMC - PMC4139059
MID - NIHMS296607
OTO - NOTNLM
OT  - Breast Cancer
OT  - Doxorubicin
OT  - Drug Delivery Nanoparticle
OT  - Magnetic Iron Oxide Nanoparticles
OT  - Targeted Nanoparticle
OT  - uPAR
EDAT- 2008/12/01 00:00
MHDA- 2008/12/01 00:01
PMCR- 2014/08/20
CRDT- 2014/08/26 06:00
PHST- 2014/08/26 06:00 [entrez]
PHST- 2008/12/01 00:00 [pubmed]
PHST- 2008/12/01 00:01 [medline]
PHST- 2014/08/20 00:00 [pmc-release]
AID - 10.1166/jbn.2008.007 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2008 Dec 1;4(4):439-449. doi: 10.1166/jbn.2008.007.