PMID- 25153703
OWN - NLM
STAT- MEDLINE
DCOM- 20150522
LR  - 20231110
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 8
DP  - 2014
TI  - Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
PG  - e104804
LID - 10.1371/journal.pone.0104804 [doi]
LID - e104804
AB  - Dendritic cells (DCs) play a major role in the innate immune response since they 
      recognize a broad repertoire of PAMPs mainly via Toll-like receptors (TLRs). 
      During Helicobacter pylori (H. pylori) infection, TLRs have been shown to be 
      important to control cytokine response particularly in murine DCs. In the present 
      study we analyzed the effect of blocking TLRs on human DCs. Co-incubation of 
      human DCs with H. pylori resulted in the release of the pro-inflammatory 
      cytokines IL-12p70, IL-6 and IL-10. Release of IL-12p70 and IL-10 was 
      predominantly influenced when TLR4 signaling was blocked by adding specific 
      antibodies, suggesting a strong influence on subsequent T cell responses through 
      TLR4 activation on DCs. Co-incubation of H. pylori-primed DC with allogeneic CD4+ 
      T cells resulted in the production of IFN-gamma and IL-17A as well as the expression 
      of Foxp3, validating a mixed Th1/Th17 and Treg response in vitro. Neutralization 
      of TLR4 during H. pylori infection resulted in significantly decreased amounts of 
      IL-17A and IFN-gamma and reduced levels of Foxp3-expressing and IL-10-secreting T 
      cells. Our findings suggest that DC cytokine secretion induced upon TLR4-mediated 
      recognition of H. pylori influences inflammatory and regulatory T cell responses, 
      which might facilitate the chronic bacterial persistence.
FAU - Kabisch, Romy
AU  - Kabisch R
AD  - Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische 
      Universitat Munchen, Munchen, Germany.
FAU - Mejias-Luque, Raquel
AU  - Mejias-Luque R
AD  - Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische 
      Universitat Munchen, Munchen, Germany.
FAU - Gerhard, Markus
AU  - Gerhard M
AD  - Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische 
      Universitat Munchen, Munchen, Germany.
FAU - Prinz, Christian
AU  - Prinz C
AD  - Lehrstuhl fur Innere Medizin 1, Universitat Witten/Herdecke, Wuppertal, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140825
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (Toll-Like Receptors)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies, Neutralizing/pharmacology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Polarity
MH  - Dendritic Cells/immunology/*microbiology
MH  - Helicobacter Infections/*immunology
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-17/metabolism
MH  - Interleukin-6/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Toll-Like Receptors/*physiology
PMC - PMC4143222
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/08/26 06:00
MHDA- 2015/05/23 06:00
PMCR- 2014/08/25
CRDT- 2014/08/26 06:00
PHST- 2014/05/10 00:00 [received]
PHST- 2014/07/14 00:00 [accepted]
PHST- 2014/08/26 06:00 [entrez]
PHST- 2014/08/26 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
PHST- 2014/08/25 00:00 [pmc-release]
AID - PONE-D-14-20029 [pii]
AID - 10.1371/journal.pone.0104804 [doi]
PST - epublish
SO  - PLoS One. 2014 Aug 25;9(8):e104804. doi: 10.1371/journal.pone.0104804. 
      eCollection 2014.