PMID- 25155198
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR  - 20180501
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 468-470
DP  - 2014 Nov
TI  - PKCdelta is required for porcine reproductive and respiratory syndrome virus 
      replication.
PG  - 96-103
LID - S0042-6822(14)00355-9 [pii]
LID - 10.1016/j.virol.2014.07.040 [doi]
AB  - Protein kinase C (PKC) that transduces signals to modulate a wide range of 
      cellular functions has been shown to regulate a number of viral infections. 
      Herein, we showed that inhibition of PKC with the PKC inhibitor GF109203X 
      significantly impaired porcine reproductive and respiratory syndrome virus 
      (PRRSV) replication. Inhibition of PKC led to virus yield reduction, which was 
      associated with decreased viral RNA synthesis and lowered virus protein 
      expression. And this inhibitory effect by PKC inhibitor was shown to occur at the 
      early stage of PRRSV infection. Subsequently, we found that PRRSV infection 
      activated PKCdelta in PAMs and knockdown of PKCdelta by small interfering RNA (siRNA) 
      suppressed PRRSV replication, suggesting that novel PKCdelta may play an important 
      factor in PRRSV replication. Taken together, these data imply that PKC is 
      involved in PRRSV infection and beneficial to PRRSV replication, extending our 
      understanding of PRRSV replication.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zhao, Haiyan
AU  - Zhao H
AD  - State Key Laboratories of Agrobiotechnology, China; Department of Microbiology 
      and Immunology, College of Biological Science, China Agricultural University, 
      Beijing 100193, China.
FAU - Guo, Xue-Kun
AU  - Guo XK
AD  - State Key Laboratories of Agrobiotechnology, China; Department of Microbiology 
      and Immunology, College of Biological Science, China Agricultural University, 
      Beijing 100193, China.
FAU - Bi, Yanmin
AU  - Bi Y
AD  - State Key Laboratories of Agrobiotechnology, China; Department of Microbiology 
      and Immunology, College of Biological Science, China Agricultural University, 
      Beijing 100193, China.
FAU - Zhu, Yihui
AU  - Zhu Y
AD  - College of Biological Science, Hunan Normal University, Changsha 410006, China.
FAU - Feng, Wen-Hai
AU  - Feng WH
AD  - State Key Laboratories of Agrobiotechnology, China; Department of Microbiology 
      and Immunology, College of Biological Science, China Agricultural University, 
      Beijing 100193, China. Electronic address: whfeng@cau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140823
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.11.13 (Protein Kinase C-delta)
RN  - L79H6N0V6C (bisindolylmaleimide I)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Viral/*physiology
MH  - Indoles/pharmacology
MH  - Macrophages, Alveolar/virology
MH  - Maleimides/pharmacology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Kinase C-delta/antagonists & inhibitors/*metabolism
MH  - RNA, Viral/genetics/metabolism
MH  - Swine
MH  - Viral Proteins/genetics/metabolism
MH  - Virus Replication/*physiology
OTO - NOTNLM
OT  - PKCdelta
OT  - PRRSV
OT  - Protein Kinase C inhibitor
OT  - Protein kinase C
OT  - siRNA
EDAT- 2014/08/27 06:00
MHDA- 2015/02/18 06:00
CRDT- 2014/08/27 06:00
PHST- 2014/05/10 00:00 [received]
PHST- 2014/06/17 00:00 [revised]
PHST- 2014/07/21 00:00 [accepted]
PHST- 2014/08/27 06:00 [entrez]
PHST- 2014/08/27 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
AID - S0042-6822(14)00355-9 [pii]
AID - 10.1016/j.virol.2014.07.040 [doi]
PST - ppublish
SO  - Virology. 2014 Nov;468-470:96-103. doi: 10.1016/j.virol.2014.07.040. Epub 2014 
      Aug 23.