PMID- 25155407
OWN - NLM
STAT- MEDLINE
DCOM- 20150918
LR  - 20231213
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 80
IP  - 3
DP  - 2014 Nov
TI  - A stearoyl-acyl carrier protein desaturase, NbSACPD-C, is critical for ovule 
      development in Nicotiana benthamiana.
PG  - 489-502
LID - 10.1111/tpj.12649 [doi]
AB  - Stearoyl-acyl carrier protein desaturase (SACPD) activity is essential for 
      production of the major unsaturated fatty acids (UFAs) in plant lipids. We report 
      here the characterization of three SACPD genes from Nicotiana benthamiana, 
      NbSACPD-A, -B, and -C. All three genes share high similarity to AtSSI2/FAB2 
      (Suppressor of Salicylic acid-Insensitivity2/Fatty Acid Biosynthesis2), the 
      primary SACPD isoform in Arabidopsis. Knocking down the expression of individual 
      or combinations of NbSACPDs by an artificial microRNA approach resulted in 
      significantly reduced accumulation of 18C UFAs and elevated levels of 18:0-FA 
      (Fatty acids) in leaves, indicating that all three genes participated in fatty 
      acid desaturation. The triple knockdown (KD) plants displayed severe growth 
      phenotypes, including spontaneous cell death and dwarfing. While no vegetative 
      morphologic abnormality was observed in NbSACPD-A, -B, or -C KD plants, 
      strikingly, NbSACPD-C KD plants produced small fruits with aborted ovules. 
      Reciprocal crosses with wild-type and NbSACPD-C KD plants revealed that knocking 
      down NbSACPD-C expression caused female, but not male, sterility. Furthermore, 
      arrested ovule development and significantly altered lipid composition in ovaries 
      were observed in NbSACPD-C KD plants, consistent with the predominant NbSACPD-C 
      expression in ovules. The ovule development defect was fully complemented by 
      coexpressing an amiRNA-resistant NbSACPD-C variant in the NbSACPD-C KD 
      background, further supporting a specific requirement for NbSACPD-C in female 
      fertility. Our results thus indicated that NbSACPD-C plays a critical role 
      maintaining membrane lipid composition in ovule development for female fertility 
      in N. benthamiana, complementing and extending prior understanding on the 
      well-demonstrated roles of SACPDs in biotic and abiotic stresses.
CI  - (c) 2014 The Authors The Plant Journal (c) 2014 John Wiley & Sons Ltd.
FAU - Zhang, Jiantao
AU  - Zhang J
AD  - Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, 24061, USA.
FAU - Li, Jianhui
AU  - Li J
FAU - Garcia-Ruiz, Hernan
AU  - Garcia-Ruiz H
FAU - Bates, Philip D
AU  - Bates PD
FAU - Mirkov, Theodore Erik
AU  - Mirkov TE
FAU - Wang, Xiaofeng
AU  - Wang X
LA  - eng
SI  - GENBANK/AAX86049
SI  - GENBANK/AAX86050
SI  - GENBANK/ABM45911
SI  - GENBANK/KJ608351
SI  - GENBANK/KJ608352
SI  - GENBANK/KJ608353
GR  - R01 GM120108/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20140919
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Fatty Acids)
RN  - 0 (MicroRNAs)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.19.- (Fatty Acid Desaturases)
RN  - EC 1.14.19.2 (acyl-(acyl-carrier-protein)desaturase)
RN  - O414PZ4LPZ (Salicylic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Fatty Acid Desaturases/genetics/metabolism
MH  - Fatty Acids/metabolism
MH  - Gene Knockdown Techniques
MH  - MicroRNAs/genetics
MH  - Mixed Function Oxygenases/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Ovule
MH  - Phenotype
MH  - Plant Leaves/genetics
MH  - Salicylic Acid/metabolism
MH  - Nicotiana/*enzymology/genetics/growth & development
OTO - NOTNLM
OT  - Nicotiana benthamiana
OT  - fatty acids
OT  - female sterility
OT  - ovule development
OT  - stearoyl-acyl carrier protein desaturase
EDAT- 2014/08/27 06:00
MHDA- 2015/09/19 06:00
CRDT- 2014/08/27 06:00
PHST- 2014/05/31 00:00 [received]
PHST- 2014/08/13 00:00 [revised]
PHST- 2014/08/18 00:00 [accepted]
PHST- 2014/08/27 06:00 [entrez]
PHST- 2014/08/27 06:00 [pubmed]
PHST- 2015/09/19 06:00 [medline]
AID - 10.1111/tpj.12649 [doi]
PST - ppublish
SO  - Plant J. 2014 Nov;80(3):489-502. doi: 10.1111/tpj.12649. Epub 2014 Sep 19.