PMID- 25155411
OWN - NLM
STAT- MEDLINE
DCOM- 20160727
LR  - 20171116
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 44
IP  - 5
DP  - 2015 May
TI  - Intra-epithelially entrapped blood vessels in ameloblastoma.
PG  - 378-85
LID - 10.1111/jop.12247 [doi]
AB  - BACKGROUND: The ameloblastoma is a benign but locally aggressive odontogenic 
      neoplasm with a high recurrence rate. While significant progress has been made in 
      our understanding regarding the role of tumoral vasculature relative to the 
      diverse behavioral characteristics of this tumor, no attention has been paid to a 
      distinct subset of blood vessels entrapped within its epithelial compartment. As 
      vascular niches are known to influence tumoral growth, clarification of these 
      vessels is important. The objectives of this study were to investigate the 
      morphologic characteristics of intra-epithelially entrapped blood vessels (IEBVs) 
      in ameloblastoma and to speculate on their relevance. MATERIALS AND METHOD: Here, 
      we evaluated the frequency, microvessel density (MVD), morphology, and 
      distribution pattern of IEBVs in 77 ameloblastoma of different subtypes based on 
      their immunoreactivity for endothelial markers (CD34, CD31, CD105), vascular 
      tight junction protein (claudin-5), pericyte [alpha-smooth muscle actin (alpha-sma)], and 
      vascular basement membrane (collagen IV). RESULTS: IEBVs were heterogeneously 
      detected in ameloblastoma. Their mean MVD (CD34 = 15.46 +/- 7.25; CD31 = 15.8 +/- 
      5.04; CD105 = 0.82 +/- 0.51) showed no significant correlation with different 
      subtypes, and between primary and recurrent tumors (P > 0.05). These microvessels 
      may occur as single/clusters of capillary sprouts, or formed compressed 
      branching/non-branching slits entrapped within the epithelial compartment, and in 
      direct apposition with polyhedral/granular neoplastic epithelial cells. They 
      expressed proteins for endothelial tight junctions (claudin-5-positive) and 
      pericytes (alpha-sma-positive) but had deficient basement membrane (collagen IV weak 
      to absent). Aberrant expression for CD34, CD31, and CD105 in tumor epithelium was 
      variably observed. CONCLUSIONS: Although rare in occurrence, identification of 
      IEBVs in ameloblastoma could potentially represent a new paradigm for vascular 
      assessment of this neoplasm.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Siar, Chong Huat
AU  - Siar CH
AD  - Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of 
      Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
FAU - Ishak, Ismadi
AU  - Ishak I
FAU - Ng, Kok Han
AU  - Ng KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140826
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (ENG protein, human)
RN  - 0 (Endoglin)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Ameloblastoma/*blood supply/*pathology
MH  - Antigens, CD
MH  - Antigens, CD34/metabolism
MH  - Child
MH  - Endoglin
MH  - Endothelium, Vascular/metabolism/*pathology
MH  - Epithelial Cells/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Jaw Neoplasms/*blood supply/*pathology
MH  - Male
MH  - Microvessels/pathology
MH  - Middle Aged
MH  - Neovascularization, Pathologic/pathology
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Receptors, Cell Surface
MH  - Young Adult
OTO - NOTNLM
OT  - ameloblastoma
OT  - endothelium
OT  - pericyte
OT  - tight junction
OT  - vascular basement membrane
EDAT- 2014/08/27 06:00
MHDA- 2016/07/28 06:00
CRDT- 2014/08/27 06:00
PHST- 2014/07/19 00:00 [accepted]
PHST- 2014/08/27 06:00 [entrez]
PHST- 2014/08/27 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - 10.1111/jop.12247 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2015 May;44(5):378-85. doi: 10.1111/jop.12247. Epub 2014 Aug 
      26.