PMID- 25156359
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20221207
IS  - 1465-3478 (Electronic)
IS  - 0963-7486 (Linking)
VI  - 65
IP  - 8
DP  - 2014 Dec
TI  - High-performance liquid chromatography LTQ-Orbitrap mass spectrometry method for 
      tomatidine and non-target metabolites quantification in organic and normal 
      tomatoes.
PG  - 942-7
LID - 10.3109/09637486.2014.950205 [doi]
AB  - Tomatoes, members of the Solanaceae plant family, produce biologically active 
      secondary metabolites, including glycoalkaloids and aglycons, which may have both 
      adverse and beneficial biological effects. A new liquid chromatography method 
      that utilized LTQ-Orbitrap MS was developed for the analysis of tomatidine, the 
      main aglycon in tomatoes. Recoveries of tomatidine were >98.3% with the relative 
      standard deviations (RSDs) below 6.1%. The limit of detection (LODs) was 
      0.0003 mg kg(-1). The limit of quantitation (LOQs) is 0.001 mg kg(-1). The linear 
      range was between with 0.0025 and 1 mg kg(-1) with an excellent correlation 
      coefficient (R(2)) equal to 0.9990. Various tomato samples were analyzed and the 
      level of tomatidine in the 11 samples analysed was higher in normal respect to 
      organic tomatoes. The capability of the set-up Full Scan LTQ-Orbitrap MS method 
      allowed us to quantified two non-target analytes. The m/z 1032 was identified as 
      dehydrotomatine, confirmed through accurate mass studies (mass error in ppm equal 
      to 1.5017) meanwhile m/z 902 as (Glc)2-Gal-Tomatidine (beta1-Tomatine) (mass error 
      in ppm equal to 2.0719).
FAU - Caprioli, Giovanni
AU  - Caprioli G
AD  - School of Pharmacy, University of Camerino , Camerino , Italy and.
FAU - Logrippo, Serena
AU  - Logrippo S
FAU - Cahill, Michael G
AU  - Cahill MG
FAU - James, Kevin J
AU  - James KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140826
PL  - England
TA  - Int J Food Sci Nutr
JT  - International journal of food sciences and nutrition
JID - 9432922
RN  - 0 (dehydrotomatine)
RN  - 2B73S48786 (tomatidine)
RN  - 31U6547O08 (Tomatine)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Fruit/*chemistry
MH  - Limit of Detection
MH  - Solanum lycopersicum/*chemistry
MH  - Mass Spectrometry
MH  - Tomatine/*analogs & derivatives/analysis
OTO - NOTNLM
OT  - Glycoalkaloid metabolites
OT  - LC-LTQ Orbitrap MS
OT  - tomatidine
OT  - tomato
EDAT- 2014/08/27 06:00
MHDA- 2016/02/13 06:00
CRDT- 2014/08/27 06:00
PHST- 2014/08/27 06:00 [entrez]
PHST- 2014/08/27 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
AID - 10.3109/09637486.2014.950205 [doi]
PST - ppublish
SO  - Int J Food Sci Nutr. 2014 Dec;65(8):942-7. doi: 10.3109/09637486.2014.950205. 
      Epub 2014 Aug 26.