PMID- 25157845
OWN - NLM
STAT- MEDLINE
DCOM- 20151209
LR  - 20240322
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 8
DP  - 2014
TI  - Influence of delivery method on neuroprotection by bone marrow mononuclear cell 
      therapy following ventral root reimplantation with fibrin sealant.
PG  - e105712
LID - 10.1371/journal.pone.0105712 [doi]
LID - e105712
AB  - The present work compared the local injection of mononuclear cells to the spinal 
      cord lateral funiculus with the alternative approach of local delivery with 
      fibrin sealant after ventral root avulsion (VRA) and reimplantation. For that, 
      female adult Lewis rats were divided into the following groups: avulsion only, 
      reimplantation with fibrin sealant; root repair with fibrin sealant associated 
      with mononuclear cells; and repair with fibrin sealant and injected mononuclear 
      cells. Cell therapy resulted in greater survival of spinal motoneurons up to four 
      weeks post-surgery, especially when mononuclear cells were added to the fibrin 
      glue. Injection of mononuclear cells to the lateral funiculus yield similar 
      results to the reimplantation alone. Additionally, mononuclear cells added to the 
      fibrin glue increased neurotrophic factor gene transcript levels in the spinal 
      cord ventral horn. Regarding the motor recovery, evaluated by the functional 
      peroneal index, as well as the paw print pressure, cell treated rats performed 
      equally well as compared to reimplanted only animals, and significantly better 
      than the avulsion only subjects. The results herein demonstrate that mononuclear 
      cells therapy is neuroprotective by increasing levels of brain derived 
      neurotrophic factor (BDNF) and glial derived neurotrophic factor (GDNF). 
      Moreover, the use of fibrin sealant mononuclear cells delivery approach gave the 
      best and more long lasting results.
FAU - Barbizan, Roberta
AU  - Barbizan R
AD  - Laboratory of Nerve Regeneration, Department of Structural and Functional 
      Biology, University of Campinas - UNICAMP, Campinas, Sao Paulo, Brazil.
FAU - Castro, Mateus V
AU  - Castro MV
AD  - Laboratory of Nerve Regeneration, Department of Structural and Functional 
      Biology, University of Campinas - UNICAMP, Campinas, Sao Paulo, Brazil.
FAU - Barraviera, Benedito
AU  - Barraviera B
AD  - Center for the Study of Venoms and Venomous Animals (CEVAP), Sao Paulo State 
      University (UNESP - Univ Estadual Paulista), Botucatu, Sao Paulo, Brazil.
FAU - Ferreira, Rui S Jr
AU  - Ferreira RS Jr
AD  - Center for the Study of Venoms and Venomous Animals (CEVAP), Sao Paulo State 
      University (UNESP - Univ Estadual Paulista), Botucatu, Sao Paulo, Brazil.
FAU - Oliveira, Alexandre L R
AU  - Oliveira AL
AD  - Laboratory of Nerve Regeneration, Department of Structural and Functional 
      Biology, University of Campinas - UNICAMP, Campinas, Sao Paulo, Brazil.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140826
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fibrin Tissue Adhesive)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cranial Nerve Injuries
MH  - Female
MH  - Fibrin Tissue Adhesive/*therapeutic use
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor/genetics/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Leukocytes, Mononuclear/*transplantation
MH  - Microglia/physiology
MH  - Nerve Tissue/transplantation
MH  - Rats, Inbred Lew
MH  - Recovery of Function
MH  - Spinal Nerve Roots/pathology
PMC - PMC4144952
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/08/27 06:00
MHDA- 2015/12/15 06:00
PMCR- 2014/08/26
CRDT- 2014/08/27 06:00
PHST- 2014/06/08 00:00 [received]
PHST- 2014/07/23 00:00 [accepted]
PHST- 2014/08/27 06:00 [entrez]
PHST- 2014/08/27 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2014/08/26 00:00 [pmc-release]
AID - PONE-D-14-25581 [pii]
AID - 10.1371/journal.pone.0105712 [doi]
PST - epublish
SO  - PLoS One. 2014 Aug 26;9(8):e105712. doi: 10.1371/journal.pone.0105712. 
      eCollection 2014.