PMID- 25160760
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20211021
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 29
IP  - 4
DP  - 2014 Aug
TI  - Association of swine influenza H1N1 pandemic virus (SIV-H1N1p) with porcine 
      respiratory disease complex in sows from commercial pig farms in Colombia.
PG  - 242-9
LID - 10.1007/s12250-014-3471-5 [doi]
AB  - Porcine respiratory disease complex (PRDC) is a serious health problem that 
      mainly affects growing and finishing pigs. PRDC is caused by a combination of 
      viral and bacterial agents, such as porcine reproductive and respiratory syndrome 
      virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), 
      Actinobacillus pleuropneumoniae (APP), Pasteurella multocida and Porcine 
      circovirus 2 (PCV2). To characterize the specific role of swine influenza virus 
      in PRDC presentation in Colombia, 11 farms from three major production regions in 
      Colombia were examined in this study. Nasal swabs, bronchial lavage and lung 
      tissue samples were obtained from animals displaying symptoms compatible with 
      SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or 
      Madin-Darby Canine Kidney (MDCK) cells. Positive isolates, identified via the 
      hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of 
      the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the 
      hemagglutinin (HA) protein led to grouping of strains into circulating viruses 
      identified during the human outbreak of 2009, classified as pandemic H1N1-2009. 
      Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were 
      obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both 
      SIV-H1N1p-negative and -positive farms, but higher levels were recorded for 
      SIV-H1N1p-positive farms. Odds ratio (OR) and P values revealed statistically 
      significant differences (p<0.05) in PRDC presentation in gilts and multiparous 
      sows of farms positive for SIV-H1N1p. Our findings indicate that positive farms 
      have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.
FAU - Jimenez, Luisa Fernanda Mancipe
AU  - Jimenez LF
AD  - Laboratory of Animal Virology, Faculty of Veterinary Medicine and Animal Science, 
      National University of Colombia, Bogota D.C., Colombia, lfmancipej@unal.edu.co.
FAU - Ramirez Nieto, Gloria
AU  - Ramirez Nieto G
FAU - Alfonso, Victor Vera
AU  - Alfonso VV
FAU - Correa, Jairo Jaime
AU  - Correa JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140808
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Circovirus/genetics/isolation & purification
MH  - Colombia/epidemiology
MH  - Dogs
MH  - Influenza A Virus, H1N1 Subtype/classification/genetics/*isolation & purification
MH  - Mycoplasma hyopneumoniae/isolation & purification
MH  - Orthomyxoviridae Infections/epidemiology/*veterinary/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification
MH  - Respiratory Tract Diseases/epidemiology/microbiology/*veterinary/virology
MH  - Swine
MH  - Swine Diseases/epidemiology/microbiology/*virology
PMC - PMC7091121
EDAT- 2014/08/28 06:00
MHDA- 2015/05/13 06:00
PMCR- 2015/08/08
CRDT- 2014/08/28 06:00
PHST- 2014/05/13 00:00 [received]
PHST- 2014/08/01 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
PHST- 2015/08/08 00:00 [pmc-release]
AID - 3471 [pii]
AID - 10.1007/s12250-014-3471-5 [doi]
PST - ppublish
SO  - Virol Sin. 2014 Aug;29(4):242-9. doi: 10.1007/s12250-014-3471-5. Epub 2014 Aug 8.