PMID- 25160914
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20150925
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Linking)
VI  - 188
DP  - 2014 Oct 20
TI  - Phosphorylation of uridine and cytidine by uridine-cytidine kinase.
PG  - 81-7
LID - S0168-1656(14)00823-2 [pii]
LID - 10.1016/j.jbiotec.2014.08.018 [doi]
AB  - Uridine 5'-monophosphate (5'-UMP) and cytidine 5'-monophosphate (5'-CMP) were 
      biosynthesized by recombinant uridine-cytidine kinase (UCK) and acetate kinase 
      (ACK). The ack and uck genes from Escherichia coli K12 and the uck1, uck2 and ack 
      genes from Lactobacillus bulgaricus ATCC 11842 were cloned and inserted into the 
      plasmid pET-28a. All of the recombinant E. coli strains were capable of 
      overexpressing UCK and ACK, which catalyzed the reaction using guanosine 
      5'-triphosphate (GTP) as a phosphate intermediate that was regenerated by ACK 
      from acetyl phosphate. The effect of several parameters, including the substrate 
      concentration, the GTP concentration, the temperature and the reaction pH, were 
      optimized. High efficiency was achieved if uridine or cytidine was phosphorylated 
      by UCK encoded by uck from E. coli and ACK encoded by ack from L. bulgaricus. The 
      maximum conversion yield of 5'-UMP and 5'-CMP was 97% at 37  degrees C and pH 7.5 when 30 
      mM uridine/cytidine and 0.5mM GTP in a total of 1 mL were used. In addition, the 
      5'-UMP and 5'-CMP products were very stable in the reaction system and did not 
      undergo significant degradation.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Qian, Yahui
AU  - Qian Y
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Ding, Qingbao
AU  - Ding Q
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Li, Yanyu
AU  - Li Y
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Zou, Zhi
AU  - Zou Z
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Yan, Bingkun
AU  - Yan B
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Ou, Ling
AU  - Ou L
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China. Electronic address: bioxianleid@163.com.
LA  - eng
PT  - Journal Article
DEP - 20140824
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 5CSZ8459RP (Cytidine)
RN  - EC 2.7.1.48 (Uridine Kinase)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - Cytidine/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Phosphorylation
MH  - Temperature
MH  - Uridine/*metabolism
MH  - Uridine Kinase/*metabolism
OTO - NOTNLM
OT  - Acetate kinase
OT  - Cytidine 5'-monophosphate
OT  - GTP-regeneration
OT  - Uridine 5'-monophosphate
OT  - Uridine-cytidine kinase
EDAT- 2014/08/28 06:00
MHDA- 2016/06/04 06:00
CRDT- 2014/08/28 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2014/08/10 00:00 [revised]
PHST- 2014/08/18 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
AID - S0168-1656(14)00823-2 [pii]
AID - 10.1016/j.jbiotec.2014.08.018 [doi]
PST - ppublish
SO  - J Biotechnol. 2014 Oct 20;188:81-7. doi: 10.1016/j.jbiotec.2014.08.018. Epub 2014 
      Aug 24.