PMID- 25161111
OWN - NLM
STAT- MEDLINE
DCOM- 20150626
LR  - 20161020
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 16
IP  - 9
DP  - 2014 Sep
TI  - Monoclonal antibodies to heat shock protein 60 induce a protective immune 
      response against experimental Paracoccidioides lutzii.
PG  - 788-95
LID - S1286-4579(14)00104-X [pii]
LID - 10.1016/j.micinf.2014.08.004 [doi]
AB  - Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. PCM is 
      primarily caused by Paracoccidioides brasiliensis and less frequently by the 
      recently described, closely related species Paracoccidioides lutzii. Current 
      treatment requires protracted administration of systemic antibiotics and relapses 
      may frequently occur despite months of initial therapy. Hence, there is a need 
      for innovative approaches to treatment. In the present study we analyzed the 
      impact of two monoclonal antibodies (mAbs) generated against Heat Shock 60 
      (Hsp60) from Histoplasma capsulatum on the interactions of P. lutzii with 
      macrophages and on the experimental P. lutzii infection. We demonstrated that the 
      Hsp60-binding mAbs labeled P. lutzii yeast cells and enhanced their phagocytosis 
      by macrophage cells. Treatment of mice with the mAbs to Hsp60 before infection 
      reduced the pulmonary fungal burden as compared to mice treated with irrelevant 
      mAb. Hence, mAbs raised to H. capsulatum Hsp60 are protective against P. lutzii, 
      including mAb 7B6 which was non-protective against H. capsulatum, suggesting 
      differences in their capacity to bind to these fungi and to be recognized by 
      macrophages. These findings indicate that mAbs raised to one dimorphic fungus may 
      be therapeutic against additional dimorphic fungi, but also suggests that 
      biological differences in diseases may influence whether a mAb is beneficial or 
      harmful.
CI  - Copyright (c) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Thomaz, Luciana
AU  - Thomaz L
AD  - Institute of Biomedical Sciences, Department of Microbiology, Brazil. Electronic 
      address: lucithomaz@usp.br.
FAU - Nosanchuk, Joshua D
AU  - Nosanchuk JD
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA; 
      Department of Microbiology and Immunology, Albert Einstein College of Medicine, 
      Bronx, NY, USA.
FAU - Rossi, Diego C P
AU  - Rossi DC
AD  - Institute of Biomedical Sciences, Department of Microbiology, Brazil.
FAU - Travassos, Luiz R
AU  - Travassos LR
AD  - Department of Microbiology, Immunology and Parasitology, Federal University of 
      Sao Paulo, Sao Paulo, Brazil.
FAU - Taborda, Carlos P
AU  - Taborda CP
AD  - Institute of Biomedical Sciences, Department of Microbiology, Brazil; Laboratory 
      of Medical Mycology IMTSP- LIM53 University of Sao Paulo, Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140824
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Chaperonin 60)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/*therapeutic use
MH  - Antigens, Fungal/immunology
MH  - Cells, Cultured
MH  - Chaperonin 60/*immunology
MH  - Cytokines/biosynthesis
MH  - Histoplasma/immunology
MH  - Liver/microbiology
MH  - Lung/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides
MH  - Paracoccidioidomycosis/drug therapy/*immunology/pathology
MH  - Spleen/microbiology
OTO - NOTNLM
OT  - Hsp60
OT  - Monoclonal antibody
OT  - Paracoccidioides lutzii
EDAT- 2014/08/28 06:00
MHDA- 2015/06/27 06:00
CRDT- 2014/08/28 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2014/07/23 00:00 [revised]
PHST- 2014/08/06 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2015/06/27 06:00 [medline]
AID - S1286-4579(14)00104-X [pii]
AID - 10.1016/j.micinf.2014.08.004 [doi]
PST - ppublish
SO  - Microbes Infect. 2014 Sep;16(9):788-95. doi: 10.1016/j.micinf.2014.08.004. Epub 
      2014 Aug 24.