PMID- 25161124
OWN - NLM
STAT- MEDLINE
DCOM- 20150609
LR  - 20181202
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 581
DP  - 2014 Oct 3
TI  - Adipose stem cells-conditioned medium blocks 6-hydroxydopamine-induced 
      neurotoxicity via the IGF-1/PI3K/AKT pathway.
PG  - 98-102
LID - S0304-3940(14)00695-8 [pii]
LID - 10.1016/j.neulet.2014.08.033 [doi]
AB  - Previous studies suggest that the delivery of neurotrophic factors secreted from 
      adipose stromal cells (ASC) protect the brain from 6-hydroxydopamine 
      (6-OHDA)-induced neurotoxicity. However, it remains unclear which secreted 
      neurotrophic factor has an important role in protecting 6-OHDA-treated neurons. 
      Through the use of antibodies in this study, we demonstrated that specific 
      neutralization of IGF-1 activity in ASC conditioned media (ASC-CM) significantly 
      blocks ASC-CM-induced neuroprotection against 6-OHDA neurotoxicity. Consistently, 
      this neuroprotection was mostly attributed to the activation of the AKT-mediated 
      signaling pathway. In contrast, brain derived neurotrophic factor (BDNF), 
      glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor 
      (VEGF) and hepatocyte growth factor (HGF) in ASC-CM did not play a role in 
      ASC-CM-induced neuroprotection against 6-OHDA.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Wang, Xianjun
AU  - Wang X
AD  - Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, 
      Shandong 276000, P.R. China.
FAU - Zhao, Zhenyu
AU  - Zhao Z
AD  - Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, 
      Shandong 276000, P.R. China.
FAU - Gong, Jian
AU  - Gong J
AD  - Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, 
      Shandong 276000, P.R. China.
FAU - Zhou, Shengnian
AU  - Zhou S
AD  - Department of Neurology, Qilu Hospital of Shandong University and Bain Science 
      Research Institute, Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 
      250012, P.R. China.
FAU - Peng, Hongjun
AU  - Peng H
AD  - Department of Pediatrics, Jinling Hospital, Nanjing University School of 
      Medicine, Nanjing 210002, P.R. China. Electronic address: hjpeng1975@hotmail.com.
FAU - Shatara, Adam
AU  - Shatara A
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Zhu, Timmy Z
AU  - Zhu TZ
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Meltzer, Rebecca
AU  - Meltzer R
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Du, Yansheng
AU  - Du Y
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Gu, Huiying
AU  - Gu H
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA. Electronic address: huiygu@iupui.edu.
LA  - eng
PT  - Journal Article
DEP - 20140823
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Antibodies)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adipocytes/cytology/*metabolism
MH  - Animals
MH  - Antibodies
MH  - Apoptosis
MH  - Cell Survival
MH  - Humans
MH  - Insulin-Like Growth Factor I/immunology/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Neurons/drug effects/*metabolism
MH  - Oxidopamine/*toxicity
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
MH  - Stromal Cells/cytology/*metabolism
OTO - NOTNLM
OT  - 6-hydroxydopamine
OT  - AKT
OT  - ASC-CM
OT  - IGF-1
OT  - Neuronal cell death
EDAT- 2014/08/28 06:00
MHDA- 2015/06/10 06:00
CRDT- 2014/08/28 06:00
PHST- 2014/05/21 00:00 [received]
PHST- 2014/07/31 00:00 [revised]
PHST- 2014/08/18 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2015/06/10 06:00 [medline]
AID - S0304-3940(14)00695-8 [pii]
AID - 10.1016/j.neulet.2014.08.033 [doi]
PST - ppublish
SO  - Neurosci Lett. 2014 Oct 3;581:98-102. doi: 10.1016/j.neulet.2014.08.033. Epub 
      2014 Aug 23.