PMID- 25161698
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140827
LR  - 20211021
IS  - 1756-8935 (Print)
IS  - 1756-8935 (Electronic)
IS  - 1756-8935 (Linking)
VI  - 7
DP  - 2014
TI  - DNA methylation dynamics during ex vivo differentiation and maturation of human 
      dendritic cells.
PG  - 21
LID - 10.1186/1756-8935-7-21 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are important mediators of innate and adaptive 
      immune responses, but the gene networks governing their lineage differentiation 
      and maturation are poorly understood. To gain insight into the mechanisms that 
      promote human DC differentiation and contribute to the acquisition of their 
      functional phenotypes, we performed genome-wide base-resolution mapping of 
      5-methylcytosine in purified monocytes and in monocyte-derived immature and 
      mature DCs. RESULTS: DC development and maturation were associated with a great 
      loss of DNA methylation across many regions, most of which occurs at predicted 
      enhancers and binding sites for known transcription factors affiliated with DC 
      lineage specification and response to immune stimuli. In addition, we discovered 
      novel genes that may contribute to DC differentiation and maturation. 
      Interestingly, many genes close to demethylated CG sites were upregulated in 
      expression. We observed dynamic changes in the expression of TET2, DNMT1, DNMT3A 
      and DNMT3B coupled with temporal locus-specific demethylation, providing possible 
      mechanisms accounting for the dramatic loss in DNA methylation. CONCLUSIONS: Our 
      study is the first to map DNA methylation changes during human DC differentiation 
      and maturation in purified cell populations and will greatly enhance the 
      understanding of DC development and maturation and aid in the development of more 
      efficacious DC-based therapeutic strategies.
FAU - Zhang, Xue
AU  - Zhang X
AD  - Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
FAU - Ulm, Ashley
AU  - Ulm A
AD  - Division of Asthma Research, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
FAU - Somineni, Hari K
AU  - Somineni HK
AD  - Division of Asthma Research, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
FAU - Oh, Sunghee
AU  - Oh S
AD  - Division of Human Genetics, Kim Sook Za Children's Hospital Medical Center 
      Research Foundation, 745 JikJi Daero Heung Deok Gu, Cheongju, Chung Buk 361-841, 
      South Korea.
FAU - Weirauch, Matthew T
AU  - Weirauch MT
AD  - Center for Autoimmune Genomics and Etiology and Division of Biomedical 
      Informatics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave, 
      Cincinnati, OH 45229, USA.
FAU - Zhang, Hong-Xuan
AU  - Zhang HX
AD  - Procter & Gamble Co., Mason Business Center, 8700 S Mason Montgomery Road, Mason, 
      OH 45040, USA.
FAU - Chen, Xiaoting
AU  - Chen X
AD  - School of Electronic and Computing Systems, University of Cincinnati, Cincinnati, 
      Ohio 45221, USA.
FAU - Lehn, Maria A
AU  - Lehn MA
AD  - Division of Immunobiology, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
FAU - Janssen, Edith M
AU  - Janssen EM
AD  - Division of Immunobiology, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
FAU - Ji, Hong
AU  - Ji H
AD  - Division of Asthma Research, Cincinnati Children's Hospital Medical Center, 3333 
      Burnet Ave, Cincinnati, OH 45229, USA.
LA  - eng
GR  - P30 AR047363/AR/NIAMS NIH HHS/United States
GR  - R01 CA138617/CA/NCI NIH HHS/United States
GR  - R21 AI101375/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20140820
PL  - England
TA  - Epigenetics Chromatin
JT  - Epigenetics & chromatin
JID - 101471619
PMC - PMC4144987
OTO - NOTNLM
OT  - DNA methylation
OT  - DNMT
OT  - Differentiation
OT  - Human dendritic cells
OT  - Maturation
OT  - Monocytes
OT  - TET
EDAT- 2014/08/28 06:00
MHDA- 2014/08/28 06:01
PMCR- 2014/08/20
CRDT- 2014/08/28 06:00
PHST- 2014/05/13 00:00 [received]
PHST- 2014/07/29 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2014/08/28 06:01 [medline]
PHST- 2014/08/20 00:00 [pmc-release]
AID - 1756-8935-7-21 [pii]
AID - 10.1186/1756-8935-7-21 [doi]
PST - epublish
SO  - Epigenetics Chromatin. 2014 Aug 20;7:21. doi: 10.1186/1756-8935-7-21. eCollection 
      2014.