PMID- 25163561
OWN - NLM
STAT- MEDLINE
DCOM- 20150707
LR  - 20211021
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 22
IP  - 2
DP  - 2015 Jan
TI  - Characterization of bacteria isolated from palaeoproterozoic metasediments for 
      sequestration of carbon dioxide and formation of calcium carbonate.
PG  - 1499-511
LID - 10.1007/s11356-014-3442-2 [doi]
AB  - Bacterial community of palaeoproterozoic metasediments was enriched in the 
      chemostat in the presence of different concentrations of NaHCO3. Six bacterial 
      isolates were isolated from the chemostat on nutrient agar plates on the basis of 
      distinct morphology. Denaturing gradient gel electrophoresis (DGGE) proved the 
      presence of six operational taxonomic units (OTUs) at 50 and 100 mM NaHCO3. The 
      OTU was reduced to three and one at enrichment concentration of 150 and 200 mM 
      NaHCO3 respectively. These six isolates were tested for sequestration of carbon 
      dioxide by (14)C metabolic labeling of NaH(14)CO3. Among the six isolates, one of 
      the bacterium showed better potency to fix radiolabeled NaH(14)CO3. The isolate 
      (ISTD04) was identified as Serratia sp. by 16S ribosomal RNA (16S rRNA) sequence 
      analysis and was found to be same as the DGGE OTU sequence at 200-mM NaHCO3 
      concentration. The bacterium was tested for product formation in form of calcite 
      crystals in presence of 5 % CO2. Scanning electron microscopy (SEM) of product 
      formed by the bacterium revealed defined faceted rhombohedral structure which 
      resembled calcite and vaterite phases of the crystal. Formation of calcium 
      carbonate crystals was further confirmed by Fourier transform infrared (FTIR) 
      spectroscopy as carbonate group showing strong vibration at 1,456 cm(-1). Major 
      calcite phase diffraction peaks were determined by X-ray diffraction (XRD) 
      analysis, and energy-dispersive X-ray (EDX) analysis showed the presence of CaO 
      (72 %) and carbon (18 %). Bacterium use bicarbonate as carbon source for their 
      growth as well as by-product formation in form of calcite shows carbon 
      circulation and storage.
FAU - Srivastava, Shaili
AU  - Srivastava S
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110 
      067, India, shailisrivastava05@gmail.com.
FAU - Bharti, Randhir K
AU  - Bharti RK
FAU - Thakur, Indu Shekhar
AU  - Thakur IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140828
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 8MDF5V39QO (Sodium Bicarbonate)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Calcium Carbonate/*metabolism
MH  - Carbon Dioxide/*metabolism
MH  - Carbon Radioisotopes/analysis
MH  - Carbon Sequestration/*physiology
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Geologic Sediments/*microbiology
MH  - Global Warming/*prevention & control
MH  - Microscopy, Electron, Scanning
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Serratia/genetics/*metabolism
MH  - Sodium Bicarbonate
MH  - *Soil Microbiology
MH  - Species Specificity
MH  - Spectroscopy, Fourier Transform Infrared
MH  - X-Ray Diffraction
EDAT- 2014/08/29 06:00
MHDA- 2015/07/08 06:00
CRDT- 2014/08/29 06:00
PHST- 2014/06/05 00:00 [received]
PHST- 2014/08/11 00:00 [accepted]
PHST- 2014/08/29 06:00 [entrez]
PHST- 2014/08/29 06:00 [pubmed]
PHST- 2015/07/08 06:00 [medline]
AID - 10.1007/s11356-014-3442-2 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2015 Jan;22(2):1499-511. doi: 
      10.1007/s11356-014-3442-2. Epub 2014 Aug 28.