PMID- 25163685 OWN - NLM STAT- MEDLINE DCOM- 20150708 LR - 20211021 IS - 1097-4547 (Electronic) IS - 0360-4012 (Print) IS - 0360-4012 (Linking) VI - 93 IP - 1 DP - 2015 Jan TI - Calcyclin-binding protein/Siah-1-interacting protein as a regulator of transcriptional responses in brain cells. PG - 75-81 LID - 10.1002/jnr.23466 [doi] AB - The calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP) is highly expressed in the brain and has been shown to regulate beta-catenin-driven transcription in thymocytes. Therefore, we investigated whether CacyBP/SIP plays a role as a transcriptional regulator in brain cells. In brain-derived neurotrophic factor (BDNF)- and forskolin-stimulated rat primary cortical neurons, overexpression of CacyBP/SIP enhanced transcriptional activity of the cAMP-response element (CRE). In addition, overexpressed CacyBP/SIP enhanced BDNF-mediated activation of the nuclear factor of activated T cells (NFAT) but not the serum response element (SRE). These stimulatory effects required an intact C-terminal domain of CacyBP/SIP. Moreover, in C6 rat glioma cells, the overexpressed CacyBP/SIP enhanced activation of CRE and NFAT following forskolin and serum stimulation, respectively. Conversely, knockdown of endogenous CacyBP/SIP reduced activation of CRE and NFAT but not of SRE. Taken together, these results indicate that CacyBP/SIP is a novel regulator of CRE- and NFAT-driven transcription. CI - (c) 2014 Wiley Periodicals, Inc. FAU - Kilanczyk, Ewa AU - Kilanczyk E AD - Kentucky Spinal Cord Injury Research Center and Department of Neurological Surgery, University of Louisville, Louisville, Kentucky; Nencki Institute of Experimental Biology, Warsaw, Poland. FAU - Filipek, Anna AU - Filipek A FAU - Hetman, Michal AU - Hetman M LA - eng GR - P30 GM103507/GM/NIGMS NIH HHS/United States GR - R01 NS073584/NS/NINDS NIH HHS/United States GR - NS073584-01/NS/NINDS NIH HHS/United States GR - 8P30GM103507/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20140828 PL - United States TA - J Neurosci Res JT - Journal of neuroscience research JID - 7600111 RN - 0 (NFATC Transcription Factors) RN - 0 (Nuclear Proteins) RN - 0 (RNA, Small Interfering) RN - 0 (enhanced green fluorescent protein) RN - 147336-22-9 (Green Fluorescent Proteins) RN - 1F7A44V6OU (Colforsin) RN - EC 2.3.1.48 (CREB-Binding Protein) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) RN - EC 2.3.2.27 (seven in absentia proteins) SB - IM MH - Animals MH - Animals, Newborn MH - CREB-Binding Protein/metabolism MH - Cells, Cultured MH - Cerebral Cortex/*cytology MH - Colforsin/pharmacology MH - Gene Expression Regulation/drug effects/*physiology MH - Green Fluorescent Proteins/metabolism MH - Mutation/genetics MH - NFATC Transcription Factors/metabolism MH - Neuroglia/drug effects/metabolism MH - Neurons/drug effects/*metabolism MH - Nuclear Proteins/genetics/*metabolism MH - RNA, Small Interfering/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Signal Transduction/drug effects/physiology MH - Transfection MH - Ubiquitin-Protein Ligases/genetics/*metabolism PMC - PMC4237629 MID - NIHMS616744 OTO - NOTNLM OT - CRE-driven transcription OT - CacyBP/SIP OT - brain development COIS- Conflict of Interests: The authors have no conflict of interests. EDAT- 2014/08/29 06:00 MHDA- 2015/07/15 06:00 PMCR- 2016/01/01 CRDT- 2014/08/29 06:00 PHST- 2014/01/29 00:00 [received] PHST- 2014/06/28 00:00 [revised] PHST- 2014/07/11 00:00 [accepted] PHST- 2014/08/29 06:00 [entrez] PHST- 2014/08/29 06:00 [pubmed] PHST- 2015/07/15 06:00 [medline] PHST- 2016/01/01 00:00 [pmc-release] AID - 10.1002/jnr.23466 [doi] PST - ppublish SO - J Neurosci Res. 2015 Jan;93(1):75-81. doi: 10.1002/jnr.23466. Epub 2014 Aug 28.