PMID- 25170812
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20240321
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 15
IP  - 9
DP  - 2014 Aug 28
TI  - Endophytic fungi from Lycium chinense Mill and characterization of two new Korean 
      records of Colletotrichum.
PG  - 15272-86
LID - 10.3390/ijms150915272 [doi]
AB  - Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in 
      southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly 
      used as oriental medicinal purposes. Endophytic fungi were isolated from surface 
      sterilized tissues and fruits of the medicinal plant in 2013 to identify the new 
      or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected 
      for molecular identification with the internal transcribed spacer (ITS) gene. 
      Phylogenetic analysis revealed that all isolates belonged to Ascomycota including 
      the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and 
      Nemania. Two Colletotrichum species were identified at the species level, using 
      three genes including internal transcribed spacer (ITS), glycerol-3-phosphate 
      dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along 
      with morphological observations. The fungal isolates, CNU122031 and CNU122032 
      were identified as Colletotrichum fructicola and C. brevisporum, respectively. 
      Morphological observations also well supported the molecular identification. C. 
      brevisporum is represented unrecorded species in Korea and C. fructicola is the 
      first record from the host plant.
FAU - Paul, Narayan Chandra
AU  - Paul NC
AD  - Division of Applied Bioscience & Biotechnology, College of Agriculture & Life 
      Sciences, Chonnam National University, Gwangju 500-757, Korea. ncpaul@jnu.ac.kr.
FAU - Lee, Hyang Burm
AU  - Lee HB
AD  - Division of Applied Bioscience & Biotechnology, College of Agriculture & Life 
      Sciences, Chonnam National University, Gwangju 500-757, Korea. hblee@jnu.ac.kr.
FAU - Lee, Ji Hye
AU  - Lee JH
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. leejh224@nate.com.
FAU - Shin, Kyu Seop
AU  - Shin KS
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. seopbee@korea.kr.
FAU - Ryu, Tae Hee
AU  - Ryu TH
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. fbxogml89@naver.com.
FAU - Kwon, Hye Ri
AU  - Kwon HR
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. hr7345@nate.com.
FAU - Kim, Yeong Kuk
AU  - Kim YK
AD  - Department of Herbal Crop Research, National Institute of Horticultural & Herbal 
      Science, Rural Development Administration (RDA), Eumseung 369-873, Korea. 
      kimyguk@korea.kr.
FAU - Youn, Young Nam
AU  - Youn YN
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. youngnam@cnu.ac.kr.
FAU - Yu, Seung Hun
AU  - Yu SH
AD  - Department of Agricultural Biology, College of Agriculture & Life Sciences, 
      Chungnam National University, Daejeon 305-764, Korea. shunyu@cnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140828
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Actins)
RN  - 0 (DNA, Intergenic)
RN  - 0 (Fungal Proteins)
RN  - EC 1.2.1.12 (Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating))
SB  - IM
MH  - Actins/genetics
MH  - Colletotrichum/classification/*genetics/isolation & purification
MH  - DNA, Intergenic/genetics
MH  - Fungal Proteins/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/genetics
MH  - Lycium/*microbiology
MH  - *Phylogeny
PMC - PMC4200795
EDAT- 2014/08/30 06:00
MHDA- 2015/05/15 06:00
PMCR- 2014/09/01
CRDT- 2014/08/30 06:00
PHST- 2014/06/06 00:00 [received]
PHST- 2014/07/29 00:00 [revised]
PHST- 2014/08/11 00:00 [accepted]
PHST- 2014/08/30 06:00 [entrez]
PHST- 2014/08/30 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - ijms150915272 [pii]
AID - ijms-15-15272 [pii]
AID - 10.3390/ijms150915272 [doi]
PST - epublish
SO  - Int J Mol Sci. 2014 Aug 28;15(9):15272-86. doi: 10.3390/ijms150915272.