PMID- 25172129
OWN - NLM
STAT- MEDLINE
DCOM- 20150902
LR  - 20171116
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 466
DP  - 2014 Dec 1
TI  - Highly sensitive electrochemical aptasensor for immunoglobulin E detection based 
      on sandwich assay using enzyme-linked aptamer.
PG  - 89-97
LID - S0003-2697(14)00352-2 [pii]
LID - 10.1016/j.ab.2014.08.019 [doi]
AB  - Here, we describe the fabrication of an electrochemical immunoglobulin E (IgE) 
      aptasensor using enzyme-linked aptamer in the sandwich assay method and thionine 
      as redox probe. In this protocol, 5'-amine-terminated IgE aptamer and thionine 
      were covalently attached on glassy carbon electrode modified with carbon 
      nanotubes/ionic liquid/chitosan nanocomposite. Furthermore, another IgE aptamer 
      was modified with biotin and enzyme horseradish peroxidase (HRP), which attached 
      to the aptamer via biotin-streptavidin interaction. Electrochemical impedance 
      spectroscopy (EIS) and cyclic voltammetry were performed at each stage of the 
      chemical modification process to confirm the resulting surface changes. The 
      presence of IgE induces the formation of a double aptamer sandwich structure on 
      the electrode, and the electrocatalytic reduction current of thionine in the 
      presence of hydrogen peroxide was measured as the sensor response. Under 
      optimized conditions and using differential pulse voltammetry as the measuring 
      technique, the proposed aptasensor showed a low detection limit (6 pM) and high 
      sensitivity (1.88 muA nM(-1)). This aptasensor also exhibited good stability and 
      high selectivity for IgE detection without an interfering effect of some other 
      proteins such as bovine serum albumin (BSA) and lysozyme. The application of the 
      aptasensor for IgE detection in human serum sample was also investigated. The 
      proposed protocol is quite promising as an alternative sandwich approach for 
      various protein assays.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Salimi, Abdollah
AU  - Salimi A
AD  - Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; 
      Research Center for Nanotechnology, University of Kurdistan, 66177-15175 
      Sanandaj, Iran. Electronic address: absalimi@uok.ac.ir.
FAU - Khezrian, Somayeh
AU  - Khezrian S
AD  - Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.
FAU - Hallaj, Rahman
AU  - Hallaj R
AD  - Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; 
      Research Center for Nanotechnology, University of Kurdistan, 66177-15175 
      Sanandaj, Iran.
FAU - Vaziry, Asaad
AU  - Vaziry A
AD  - Department of Animal Science, Faculty of Agricultural Science, University of 
      Kurdistan, 66177-15175 Sanandaj, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140827
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Aptamers, Nucleotide)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Animals
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biological Assay/*methods
MH  - Cattle
MH  - *Electrochemical Techniques
MH  - Horseradish Peroxidase/metabolism
MH  - Humans
MH  - Immunoglobulin E/*analysis/chemistry
MH  - Limit of Detection
MH  - Microscopy, Electron, Scanning
MH  - Multiple Myeloma/diagnosis
MH  - Serum Albumin, Bovine/chemistry
OTO - NOTNLM
OT  - Carbon nanotubes
OT  - Chitosan
OT  - Electrochemical aptasensor
OT  - Enzyme-linked sandwich assay
OT  - IgE
OT  - Ionic liquid
EDAT- 2014/08/31 06:00
MHDA- 2015/09/04 06:00
CRDT- 2014/08/31 06:00
PHST- 2014/04/05 00:00 [received]
PHST- 2014/08/18 00:00 [revised]
PHST- 2014/08/19 00:00 [accepted]
PHST- 2014/08/31 06:00 [entrez]
PHST- 2014/08/31 06:00 [pubmed]
PHST- 2015/09/04 06:00 [medline]
AID - S0003-2697(14)00352-2 [pii]
AID - 10.1016/j.ab.2014.08.019 [doi]
PST - ppublish
SO  - Anal Biochem. 2014 Dec 1;466:89-97. doi: 10.1016/j.ab.2014.08.019. Epub 2014 Aug 
      27.