PMID- 25173856
OWN - NLM
STAT- MEDLINE
DCOM- 20150114
LR  - 20181202
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 499
DP  - 2014 Nov 15
TI  - Detecting clothianidin residues in environmental and agricultural samples using 
      rapid, sensitive enzyme-linked immunosorbent assay and gold immunochromatographic 
      assay.
PG  - 1-6
LID - S0048-9697(14)01196-6 [pii]
LID - 10.1016/j.scitotenv.2014.08.029 [doi]
AB  - Two rapid, sensitive immunoassays based on monoclonal antibody for detecting 
      clothianidin were developed and applied in agricultural samples: a quantitative 
      enzyme-linked immunosorbent assay (ELISA) and a semiquantitative gold 
      immunochromatographic assay (GICA). Under optimal conditions, the half-maximal 
      inhibition concentration (IC50) and the limit of detection (LOD, IC10) of 
      clothianidin were 25.6 and 3.8 ng mL(-1) for ELISA. GICA using colloidal gold-MAb 
      probe had a visual detection limit of 8 ng mL(-1), and the results can be judged 
      by the naked eye within 10 min. The cross-reactivities of the immunoassays with 
      its analogues were negligible except for that with dinotefuran. For the spiked 
      agricultural samples, recoveries of 78.0 to 114.5% with relative standard 
      deviations (RSDs) of 3.2 to 12.8% were achieved for ELISA and further evaluated 
      by GICA. Furthermore, the results of ELISA and GICA for the authentic samples 
      correlated well with those obtained by HPLC. Overall, the proposed ELISA and GICA 
      are satisfactory for rapid, sensitive, and quantitative/semiquantitative 
      detection of clothianidin residues in agricultural samples.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Li, Ming
AU  - Li M
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
FAU - Hua, Xiude
AU  - Hua X
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
FAU - Ma, Ming
AU  - Ma M
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
FAU - Liu, Jisong
AU  - Liu J
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
FAU - Zhou, Liangliang
AU  - Zhou L
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
FAU - Wang, Minghua
AU  - Wang M
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Key Laboratory of Integrated Management of 
      Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China. 
      Electronic address: wangmha@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140828
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Guanidines)
RN  - 0 (Neonicotinoids)
RN  - 0 (Pesticide Residues)
RN  - 0 (Soil Pollutants)
RN  - 0 (Thiazoles)
RN  - 2V9906ABKQ (clothianidin)
SB  - IM
MH  - Agriculture
MH  - Chromatography, Affinity
MH  - Environmental Monitoring/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Guanidines/*analysis
MH  - Neonicotinoids
MH  - Pesticide Residues/*analysis
MH  - Soil Pollutants/*analysis
MH  - Thiazoles/*analysis
OTO - NOTNLM
OT  - Agricultural samples
OT  - Clothianidin
OT  - Enzyme-linked immunosorbent assay
OT  - Gold immunochromatographic assay
OT  - Monoclonal antibody
EDAT- 2014/09/01 06:00
MHDA- 2015/01/15 06:00
CRDT- 2014/09/01 06:00
PHST- 2014/06/30 00:00 [received]
PHST- 2014/08/08 00:00 [revised]
PHST- 2014/08/08 00:00 [accepted]
PHST- 2014/09/01 06:00 [entrez]
PHST- 2014/09/01 06:00 [pubmed]
PHST- 2015/01/15 06:00 [medline]
AID - S0048-9697(14)01196-6 [pii]
AID - 10.1016/j.scitotenv.2014.08.029 [doi]
PST - ppublish
SO  - Sci Total Environ. 2014 Nov 15;499:1-6. doi: 10.1016/j.scitotenv.2014.08.029. 
      Epub 2014 Aug 28.