PMID- 25174446
OWN - NLM
STAT- MEDLINE
DCOM- 20150518
LR  - 20231213
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 15
DP  - 2014 Aug 31
TI  - An increase of CD83+ dendritic cells ex vivo correlates with increased regulatory 
      T cells in patients with active eosinophilic granulomatosis and polyangiitis.
PG  - 32
LID - 10.1186/s12865-014-0032-5 [doi]
LID - 32
AB  - BACKGROUND: Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare 
      disease characterized by the presence of allergic granulomatosis and necrotizing 
      vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. 
      Dendritic cells (DCs) are not only critical for the induction of primary immune 
      responses; they may also be important for the induction of immunological 
      tolerance and the regulation of the type of T-cell-mediated immune response. To 
      investigate whether DC maturation is associated with EGPA disease status, we 
      examined the relationship between the maturation of DCs and the differentiation 
      of regulatory T (Treg) cells in EGPA patients. We exposed the CD14+ blood 
      monocytes of 19 patients with EGPA in remission or relapse to stimulation with 
      GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature CD83+ 
      DCs and immature CD206+ DCs. Using immunohistochemistry, we examined four 
      patients for the presence of CD83+ and CD206+ DCs in the lung at the onset of 
      EGPA. RESULTS: The percentage of CD83+ cells among DCs differentiated from CD14+ 
      monocytes was lower for EGPA patients in relapse than in remission. The 
      percentage of CD83+ DCs was inversely correlated with the percentage of CD206+ 
      DCs and was significantly correlated with the numbers of naturally occurring CD4+ 
      regulatory Treg (nTreg; FOXP3+CD4+) cells and inducible Treg (iTreg; CD4+CD25+ T 
      cells producing IL-10 or TGF-beta) cells but not the number of eosinophils. The 
      percentage of CD206+ DCs was significantly inversely correlated with the 
      percentages of nTreg and iTreg cells but not the number of eosinophils. 
      Immunohistochemistry revealed both CD206+ DCs and CD83+ DCs in alveoli and 
      interstitial spaces at the onset of EGPA. CONCLUSION: The maturation of DCs from 
      monocytes was related to disease activity in patients with EGPA. Increased CD83+ 
      DCs in EGPA patients may induce the differentiation of iTreg and nTreg cells, 
      thereby suppressing inflammation and disease activity.
FAU - Tsurikisawa, Naomi
AU  - Tsurikisawa N
AD  - Departments of Allergy and Respirology, 18-1 Sakuradai, Minami-ku Sagamihara 
      252-0392, Kanagawa, Japan. n-tsurikisawa@sagamihara-hosp.gr.jp.
FAU - Saito, Hiroshi
AU  - Saito H
FAU - Oshikata, Chiyako
AU  - Oshikata C
FAU - Tsuburai, Takahiro
AU  - Tsuburai T
FAU - Ishiyama, Miyako
AU  - Ishiyama M
FAU - Mitomi, Hiroyuki
AU  - Mitomi H
FAU - Akiyama, Kazuo
AU  - Akiyama K
LA  - eng
PT  - Journal Article
DEP - 20140831
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Biopsy
MH  - Cell Count
MH  - Cell Differentiation/immunology
MH  - Churg-Strauss Syndrome/*immunology/pathology
MH  - Dendritic Cells/*pathology
MH  - Eosinophilic Granuloma/*immunology/pathology
MH  - Female
MH  - Humans
MH  - Immunoglobulins/*metabolism
MH  - Immunohistochemistry
MH  - Interleukin-10/biosynthesis
MH  - Lung/immunology/pathology
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Middle Aged
MH  - Monocytes/cytology
MH  - Recurrence
MH  - Remission Induction
MH  - T-Lymphocytes, Regulatory/*immunology/pathology
MH  - CD83 Antigen
PMC - PMC4159546
EDAT- 2014/09/02 06:00
MHDA- 2015/05/20 06:00
PMCR- 2014/08/31
CRDT- 2014/09/02 06:00
PHST- 2014/03/10 00:00 [received]
PHST- 2014/08/08 00:00 [accepted]
PHST- 2014/09/02 06:00 [entrez]
PHST- 2014/09/02 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PHST- 2014/08/31 00:00 [pmc-release]
AID - s12865-014-0032-5 [pii]
AID - 32 [pii]
AID - 10.1186/s12865-014-0032-5 [doi]
PST - epublish
SO  - BMC Immunol. 2014 Aug 31;15:32. doi: 10.1186/s12865-014-0032-5.