PMID- 25174942
OWN - NLM
STAT- MEDLINE
DCOM- 20150609
LR  - 20220113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 10
IP  - 5
DP  - 2014 Nov
TI  - Effects of lentiviral infection of mesenchymal stem cells on the expression of 
      octamer transcription factor 4.
PG  - 2249-54
LID - 10.3892/mmr.2014.2505 [doi]
AB  - The present study aimed to investigate the effects of lentiviral infection of 
      human umbilical cord mesenchymal stem cells (hUCMSCs) on the expression of 
      octamer transcription factor 4 (Oct4). hUCMSCs were infected with lentivirus 
      carrying the green fluorescent protein gene (GFP) at different multiplicities of 
      infection (MOI), and the optimal MOI was determined by flow cytometry; the 
      proliferation of non‑infected and GFP-carrying lentivirus‑infected hUCMSCs was 
      evaluated by the MTT assay; and the expression of the Oct4 gene was measured by 
      quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and 
      immunofluorescence staining in hUCMSCs cultured in vitro for eight weeks. 
      Positive GFP staining of hUCMSCs was estimated at >75% at 48 h following 
      infection with the GFP-carrying lentivirus (MOI=20); no effect on hUCMSC 
      proliferation was detected by the MTT assay following the infection; 
      immunofluorescence analysis detected positive Oct4 expression in the cell nuclei 
      at two and eight weeks of culture, while the relative expression of Oct4 assessed 
      by qRT-PCR was 0.9075+/-0.0124. The GFP gene carried by the lentivirus was 
      successfully expressed in hUCMSCs and had no significant effect on Oct4 
      expression, which lays a solid foundation for future studies investigating gene 
      functions via the use of exogenous markers.
FAU - Chang, Jing
AU  - Chang J
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Tang, Li
AU  - Tang L
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Lei, Han
AU  - Lei H
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Zhang, Xiao-Gang
AU  - Zhang XG
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Zuo, Zhong
AU  - Zuo Z
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Huang, Wei
AU  - Huang W
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Fu, Hang
AU  - Fu H
AD  - Department of Cardiology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140821
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (MIRN302A microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Cell Proliferation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Lentivirus/*physiology
MH  - Lentivirus Infections/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism/virology
MH  - MicroRNAs/genetics
MH  - Octamer Transcription Factor-3/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Transduction, Genetic
PMC - PMC4199401
EDAT- 2014/09/02 06:00
MHDA- 2015/06/10 06:00
PMCR- 2014/08/21
CRDT- 2014/09/02 06:00
PHST- 2013/09/04 00:00 [received]
PHST- 2014/04/16 00:00 [accepted]
PHST- 2014/09/02 06:00 [entrez]
PHST- 2014/09/02 06:00 [pubmed]
PHST- 2015/06/10 06:00 [medline]
PHST- 2014/08/21 00:00 [pmc-release]
AID - mmr-10-05-2249 [pii]
AID - 10.3892/mmr.2014.2505 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Nov;10(5):2249-54. doi: 10.3892/mmr.2014.2505. Epub 2014 Aug 
      21.