PMID- 25175178
OWN - NLM
STAT- MEDLINE
DCOM- 20150601
LR  - 20211203
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 32
IP  - 4
DP  - 2014 Oct
TI  - YAP is overexpressed in clear cell renal cell carcinoma and its knockdown reduces 
      cell proliferation and induces cell cycle arrest and apoptosis.
PG  - 1594-600
LID - 10.3892/or.2014.3349 [doi]
AB  - Yes-associated protein (YAP) has been reported to be an oncogene in a number of 
      malignancies. It constitutes an important regulatory mechanism for the Hippo 
      pathway, a key regulator of cell growth and apoptosis. The present study aimed to 
      investigate the clinical significance and the role of YAP in the development of 
      clear cell renal cell carcinoma (ccRCC). YAP expression levels were compared 
      between ccRCC and adjacent normal renal tissues by RT-PCR and 
      immunohistochemistry, respectively. YAP expression levels were then detected in 
      ccRCC cell lines 786-0 and ACHN, as well as in human embryonic kidney 293 cells 
      (HEK-293) using western blotting. Three specific YAP-shRNA lentiviral vectors 
      were constructed and transfected into 786-0 cells, and then the mRNA and protein 
      levels of YAP and downstream transcription factor TEAD1 were detected. Finally, 
      the effects of YAP silencing on proliferation and the cell cycle distribution of 
      786-0 cells were detected by Cell Counting Kit-8 (CCK-8) and flow cytometry 
      (FCM), respectively. The apoptosis rate was also analyzed by FCM. It was observed 
      that the expression levels of YAP mRNA and protein in ccRCC tissues were higher 
      than these levels in the adjacent normal renal tissues. The expression of YAP 
      protein in ccRCC tissues was significantly correlated with clinical stage and 
      differentiation. The YAP protein levels in the two ccRCC cell lines 786-0 and 
      ACHN were significantly higher than that in the HEK-293 cells. Additionally, 
      treatment of 786-0 cells with YAP-shRNA lentiviral vectors significantly reduced 
      the expression levels of YAP and TEAD1 mRNA and protein. Further analyses in 
      786-0 cells in which YAP was decreased, revealed that cell proliferation was 
      inhibited, cell cycle was arrested at the G1 phase and apoptosis was increased. 
      These results indicate that YAP is an underlying oncogene in ccRCC and it may be 
      a promising biomarker and therapeutic target of ccRCC.
FAU - Cao, Jian-Jia
AU  - Cao JJ
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Zhao, Xiu-Min
AU  - Zhao XM
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Wang, De-Lin
AU  - Wang DL
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Chen, Ke-Hong
AU  - Chen KH
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Sheng, Xia
AU  - Sheng X
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Li, Wen-Bin
AU  - Li WB
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Li, Mei-Cai
AU  - Li MC
AD  - Department of Urology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, P.R. China.
FAU - Liu, Wu-Jiang
AU  - Liu WJ
AD  - Institute of Urology, First Hospital of Peking University, Beijing, P.R. China.
FAU - He, Jiang
AU  - He J
AD  - Gastroenterology and Neurology Center, University-Town Hospital of Chongqing 
      Medical University, Chongqing, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140722
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Apoptosis/*genetics
MH  - Carcinoma, Renal Cell/*genetics/metabolism/pathology
MH  - Case-Control Studies
MH  - Cell Cycle Checkpoints/*genetics
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - Kidney Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Phosphoproteins
MH  - RNA, Messenger/*genetics
MH  - Transcription Factors
MH  - YAP-Signaling Proteins
EDAT- 2014/09/02 06:00
MHDA- 2015/06/02 06:00
CRDT- 2014/09/02 06:00
PHST- 2014/05/06 00:00 [received]
PHST- 2014/06/23 00:00 [accepted]
PHST- 2014/09/02 06:00 [entrez]
PHST- 2014/09/02 06:00 [pubmed]
PHST- 2015/06/02 06:00 [medline]
AID - 10.3892/or.2014.3349 [doi]
PST - ppublish
SO  - Oncol Rep. 2014 Oct;32(4):1594-600. doi: 10.3892/or.2014.3349. Epub 2014 Jul 22.