PMID- 25175735
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20211203
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 32
IP  - 5
DP  - 2014 Nov
TI  - Mammalian target of rapamycin signaling is involved in the vasculogenic mimicry 
      of glioma via hypoxia-inducible factor-1alpha.
PG  - 1973-80
LID - 10.3892/or.2014.3454 [doi]
AB  - The mammalian target of rapamycin (mTOR) is a crucial regulator in malignant 
      gliomas. Vasculogenic mimicry (VM) describes functional channels established by 
      highly malignant tumor cells that is different from endothelium-lined blood 
      vessels. Our previous studies confirmed the existence and clinical significance 
      of VM in medulloblastoma and glioblastoma. In the present study, by 
      immunohistochemical and CD34/PAS histochemical double-staining, 34 cases (26.8%) 
      with VM structures were identified among a total of 127 glioma cases, and these 
      VM structures were associated with mTOR expression in the glioma specimens. 
      In vitro, U87 malignant glioblastoma cells formed tube structures similar to 
      HUVECs on Matrigel in 3D culture, and mTOR-specific inhibitor rapamycin inhibited 
      VM formation in the U87 malignant glioblastoma cells under both normoxia and 
      hypoxia. In addition, rapamycin and mTOR siRNA inhibited molecules in the 
      signaling cascade of VM formation, particularly HIF-1alpha. Taken together, our 
      results demonstrated that mTOR signaling is involved in VM formation, and may be 
      a potential therapeutic target for gliomas.
FAU - Huang, Min
AU  - Huang M
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Ke, Yiquan
AU  - Ke Y
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Sun, Xinlin
AU  - Sun X
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Yu, Li
AU  - Yu L
AD  - Department of Pathology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510280, P.R. China.
FAU - Yang, Zhilin
AU  - Yang Z
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Zhang, Yonghong
AU  - Zhang Y
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Du, Mouxuan
AU  - Du M
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Wang, Jihui
AU  - Wang J
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Liu, Xiao
AU  - Liu X
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
FAU - Huang, Shuyun
AU  - Huang S
AD  - The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong 
      Province, Guangdong Provincial Key Laboratory on Brain Function Repair and 
      Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510280, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140829
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Hypoxia/drug effects
MH  - Cell Line, Tumor
MH  - Female
MH  - Glioma/*blood supply/metabolism/*pathology
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Middle Aged
MH  - Neovascularization, Pathologic/metabolism/*pathology
MH  - *Signal Transduction/drug effects
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2014/09/02 06:00
MHDA- 2015/10/27 06:00
CRDT- 2014/09/02 06:00
PHST- 2014/07/11 00:00 [received]
PHST- 2014/08/14 00:00 [accepted]
PHST- 2014/09/02 06:00 [entrez]
PHST- 2014/09/02 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - 10.3892/or.2014.3454 [doi]
PST - ppublish
SO  - Oncol Rep. 2014 Nov;32(5):1973-80. doi: 10.3892/or.2014.3454. Epub 2014 Aug 29.